Team:IvyTech-South Bend/Parts

Designed by IvyTech_South-Bend Team Group: iGEM10_IvyTechSBend-IN-USA (2010-10-26) From partsregistry.org

T9002 - LacZ

The T9002 AHL detector was ligated to a LacZ reporter gene by the conventional biobrick assembly #10.

Sequence and Features Format: Subparts|Ruler|SS|DSSearch: Length: 3154 bpContext: Part onlyGet selected sequence . .1 11 21 31 41 51 61 71 81 91 1 500 1K 1.5K 2K 2.5K 3K 3.5K 4K T9002 I732094 E0044 C0062 E0040 I732006 B0010 B0010 B0010 R0062 R0040 B0012 B0012 B0012 I732007 B0032 B0034 B0034 B0034 prefix luxR lacZ STOP Stop Stop Stop -35 -10 -35 -10 RBS-3Weak RBS LuxR/HSL TetR 1 TetR 2 T7 TE T7 TE T7 TE BglII BamHI PolyA PolyA PolyA T A START tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag agaaagagga gaaatactag atgaaaaaca taaatgccga 1 agggatagtc actatctcta actgtaggga tagtcactat ctctatgact cgtgatgatc tctttctcct ctttatgatc tactttttgt atttacggct T9002 C0062 R0040 B0034 prefix luxR -35 -10 TetR 1 TetR 2 cgacacatac agaataatta ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta aaatggtaca ttgtgaatat 101 gctgtgtatg tcttattaat tattttaatt tcgaacatct tcgttattac tataattagt tacgaataga ctatactgat tttaccatgt aacacttata T9002 C0062 luxR tatttactcg cgatcattta tcctcattct atggttaaat ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 201 ataaatgagc gctagtaaat aggagtaaga taccaattta gactataaag ttaggatcta ttaatgggat tttttacctc cgttataata ctactgcgat T9002 C0062 luxR T atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc 301 taaattattt tatactagga tatcatctaa taagattgag gttagtaagt ggttaattaa ccttatataa acttttgtta cgacatttat tttttagagg T9002 C0062 luxR aaatgtaatt aaagaagcga aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg gaatgcttag ttttgcacat 401 tttacattaa tttcttcgct tttgtagtcc agaatagtga cccaaatcaa agggataagt atgccgattg ttaccgaagc cttacgaatc aaaacgtgta T9002 C0062 luxR tcagaaaaag acaactatat agatagttta tttttacatg cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 501 agtctttttc tgttgatata tctatcaaat aaaaatgtac gcacatactt gtatggtaat taacaaggaa gagatcaact attaatagct ttttatttat T9002 C0062 luxR A tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg catgcgaagg aaaaagctct tgggatattt caaaaatatt 601 atcgtttatt atttagtttg ttgctaaatt ggttttctct ttttcttaca aatcgcaccc gtacgcttcc tttttcgaga accctataaa gtttttataa T9002 C0062 luxR aggttgcagt gagcgtactg tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta tttctaaagc aattttaaca 701 tccaacgtca ctcgcatgac agtgaaaggt aaattggtta cgcgtttact ttgagttatg ttgtttggcg acggtttcat aaagatttcg ttaaaattgt T9002 C0062 luxR ggagcaattg attgcccata ctttaaaaat taataacact gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 801 cctcgttaac taacgggtat gaaattttta attattgtga ctatcacgat cacatctagt gatgatctcg gtccgtagtt tattttgctt tccgagtcag T9002 C0062 B0010 luxR gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata 901 ctttctgacc cggaaagcaa aatagacaac aaacagccac ttgcgagaga tgatctcagt gtgaccgagt ggaagcccac ccggaaagac gcaaatatat T9002 B0010 B0012 Stop T7 TE PolyA ctagagacct gtaggatcgt acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagagt cacacaggaa agtactagat gcgtaaagga 1001 gatctctgga catcctagca tgtccaaatg cgttctttta ccaaacaata tcagcttatt tatgatctca gtgtgtcctt tcatgatcta cgcatttcct T9002 E0040 R0062 B0032 -35 -10 RBS-3Weak RBS LuxR/HSL gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa 1101 cttcttgaaa agtgacctca acagggttaa gaacaactta atctaccact acaattaccc gtgtttaaaa gacagtcacc tctcccactt ccactacgtt T9002 E0040 catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt 1201 gtatgccttt tgaatgggaa tttaaataaa cgtgatgacc ttttgatgga caaggtaccg gttgtgaaca gtgatgaaag ccaataccac aagttacgaa T9002 E0040 tgcgagatac ccagatcata tgaaacagca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg 1301 acgctctatg ggtctagtat actttgtcgt actgaaaaag ttctcacggt acgggcttcc aatacatgtc ctttcttgat ataaaaagtt tctactgccc T9002 E0040 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg 1401 ttgatgttct gtgcacgact tcagttcaaa cttccactat gggaacaatt atcttagctc aattttccat aactaaaatt tcttctacct ttgtaagaac T9002 E0040 gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat 1501 ctgtgtttaa ccttatgttg atattgagtg tgttacatat gtagtaccgt ctgtttgttt tcttacctta gtttcaattg aagttttaat ctgtgttgta T9002 E0040 tgaagatgga agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa 1601 acttctacct tcgcaagttg atcgtctggt aatagttgtt ttatgaggtt aaccgctacc gggacaggaa aatggtctgt tggtaatgga caggtgtgtt T9002 E0040 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca 1701 agacgggaaa gctttctagg gttgcttttc tctctggtgt accaggaaga actcaaacat tgtcgacgac cctaatgtgt accgtaccta cttgatatgt T9002 E0040 aataataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact 1801 ttattattat gatctcggtc cgtagtttat tttgctttcc gagtcagctt tctgacccgg aaagcaaaat agacaacaaa cagccacttg cgagagatga T9002 E0040 B0010 agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta gaggaagatc ttccggatcc ggtactagag aaagaggaga aatactagat 1901 tctcagtgtg accgagtgga agcccacccg gaaagacgca aatatatgat ctccttctag aaggcctagg ccatgatctc tttctcctct ttatgatcta T9002 I732094 I732006 B0012 I732007 B0034 lacZ Stop T7 TE BglII BamHI PolyA START gaccatgatt acggattcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 2001 ctggtactaa tgcctaagtg accggcagca aaatgttgca gcactgaccc ttttgggacc gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag I732094 I732006 lacZ START gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatggcgctt tgcctggttt ccggcaccag 2101 cggtcgaccg cattatcgct tctccgggcg tggctagcgg gaagggttgt caacgcgtcg gacttaccgc ttaccgcgaa acggaccaaa ggccgtggtc I732094 I732006 lacZ aagcggtgcc ggaaagctgg ctggagtaat aatactagag aaagaggaga aatactagat gcgtaaagga gaagaacttt tcactggagt tgtcccaatt 2201 ttcgccacgg cctttcgacc gacctcatta ttatgatctc tttctcctct ttatgatcta cgcatttcct cttcttgaaa agtgacctca acagggttaa I732094 E0044 I732006 B0034 lacZ STOP cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt aaatttattt 2301 gaacaactta atctaccact acaattaccc gtgtttaaaa gacagtcacc tctcccactt ccactacgtt gtatgccttt tgaatgggaa tttaaataaa I732094 E0044 gcactactgg aaaactacct gttccatggc caacacttgt cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 2401 cgtgatgacc ttttgatgga caaggtaccg gttgtgaaca gtgatgaaag ccaataccac aagttacgaa acgctctatg ggtctagtat actttgtcgt I732094 E0044 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt 2501 actgaaaaag ttctcacggt acgggcttcc aatacatgtc ctttcttgat ataaaaagtt tctactgccc ttgatgttct gtgcacgact tcagttcaaa I732094 E0044 gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac tataactcac 2601 cttccactat gggaacaatt atcttagctc aattttccat aactaaaatt tcttctacct ttgtaagaac ctgtgtttaa ccttatgttg atattgagtg I732094 E0044 acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 2701 tgttacatat gtagtaccgt ctgtttgttt tcttacctta gtttcaattg aagttttaat ctgtgttgta acttctacct tcgcaagttg atcgtctggt I732094 E0044 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag 2801 aatagttgtt ttatgaggtt aaccgctacc gggacaggaa aatggtctgt tggtaatgga caggtgtgtt agacgggaaa gctttctagg gttgcttttc I732094 E0044 agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc tgcaaacgac gaaaactacg 2901 tctctggtgt accaggaaga actcaaacat tgtcgacgac cctaatgtgt accgtaccta cttgatatgt tttccggacg acgtttgctg cttttgatgc I732094 E0044 ctgctgctgt ttaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 3001 gacgacgaca aattattatg atctcggtcc gtagtttatt ttgctttccg agtcagcttt ctgacccgga aagcaaaata gacaacaaac agccacttgc I732094 E0044 B0010 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata 3101 gagagatgat ctcagtgtga ccgagtggaa gcccacccgg aaagacgcaa atat I732094 B0010 B0012 Stop T7 TE PolyA

p(tetR)

R0040

.

B0034

luxr

C0062

.

B0010

.

B0012

lux pR

R0062

.

B0032

GFP

E0040

.

B0010

.

B0012

Adapter

I732007

.

B0034

lacZ-alpha

I732006

.

B0034

.

E0044

.

B0010

.

B0012

Assembly Compatibility:

   * 10
     COMPATIBLE WITH RFC[10]
   * 12
     COMPATIBLE WITH RFC[12]
   * 21
     INCOMPATIBLE WITH RFC[21]
     Illegal BglII site found at 1956
     Illegal BamHI site found at 1965
   * 23
     COMPATIBLE WITH RFC[23]
   * 25
     COMPATIBLE WITH RFC[25]

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