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Team:Mexico-UNAM-CINVESTAV/Notebook/Week Three
From 2010.igem.org
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==='''We were looking for a nanodrop to quantify DNA’s concentrations'''=== | ==='''We were looking for a nanodrop to quantify DNA’s concentrations'''=== | ||
==='''and tried to make ligations Psb1C3 with each one of primers'''=== | ==='''and tried to make ligations Psb1C3 with each one of primers'''=== | ||
| - | ==='''(our this year biobricks).'''=== | + | ==='''(our this year's biobricks).'''=== |
==''Tuesday''== | ==''Tuesday''== | ||
Revision as of 17:39, 26 October 2010
Week #3
20th September -24th September 2010
Monday
Planned and prepared for tomorrow's electroporation
- Depart electroporator.
- Made up LB agar plates and cloramphenicol 35ng/ml.
- We did a plan to transform Psb1C3 from plate.
We were looking for a nanodrop to quantify DNA’s concentrations
and tried to make ligations Psb1C3 with each one of primers
(our this year's biobricks).
Tuesday
Transformed DH5α competent cells, and leave 10 plates incubating 37º overnight.
- Prepared solutions for miniprep via Alcalin lisis method.
Wednsday
Results previous day transformations just two plates were completed sucessfully.
- We leave LB liquid medium at 37º overnight 4 falcon tubes each one with 30ml inoculum
Thursday
The inoculum for miniprep we prepared 6 vector’s vials
without positive results. Maybe the solutions for alcaline
lisis are not well.
- We have discused over how to quantify our cold sistem response
we gonig to use a promoter sistem, the funcional casset using J13002 as follow
- Promoter + our functional secuence + GFP
- Promoter + our functional secuence + AFP (Anty freeze protein)
- We have had a meeting with our Genomics frend’s we going to get a
colaboration on Modeling and probably at wet lab too.
