Team:RMIT Australia/Project
From 2010.igem.org
(Difference between revisions)
(→Project Details) |
(→Making the Inducible T7 Expression Vector) |
||
Line 429: | Line 429: | ||
=== Making the Inducible T7 Expression Vector === | === Making the Inducible T7 Expression Vector === | ||
+ | |||
+ | The aim for this section is to create a an inducible expression backbone. this will be achieved by altering the backbone biobrick '''BBa_J04500''', by removing the -35 and -10 elements (promoter) and inserting the bacteriophage T7 promoter. the strategy used to will be Quikchange XL mutagenesis to alter the promoter elements into restriction sites: | ||
+ | * -35 --> AflII | ||
+ | * -10 --> BamH1 | ||
+ | |||
+ | These restriction sites are NOT found in the BBa_J04500 part or vector (pSB1A2), so there will be no cross cutting. | ||
== Results == | == Results == |
Revision as of 21:14, 6 July 2010
![](https://2010.igem.org/Image:RMIT_logo.jpg)
![](https://static.igem.org/mediawiki/2008/e/ef/Navi_bg.gif)
Overall project
Your abstract
Project Details
Creation of the Taq Polymerase Part
Making the Inducible T7 Expression Vector
The aim for this section is to create a an inducible expression backbone. this will be achieved by altering the backbone biobrick BBa_J04500, by removing the -35 and -10 elements (promoter) and inserting the bacteriophage T7 promoter. the strategy used to will be Quikchange XL mutagenesis to alter the promoter elements into restriction sites:
- -35 --> AflII
- -10 --> BamH1
These restriction sites are NOT found in the BBa_J04500 part or vector (pSB1A2), so there will be no cross cutting.