From 2010.igem.org
(Difference between revisions)
|
|
| (6 intermediate revisions not shown) |
| Line 1: |
Line 1: |
| - | {{Team:Alberta/Head}}
| |
| | | | |
| - | {{Team:Alberta/navbar|project=selected}}
| |
| - |
| |
| - | {{Team:Alberta/beginLeftSideBar}}
| |
| - | {{Team:Alberta/endLeftSideBar}}
| |
| - |
| |
| - | {{Team:Alberta/beginRightSideBar}}
| |
| - | {{Team:Alberta/endRightSideBar}}
| |
| - |
| |
| - | {{Team:Alberta/beginMainContent}}
| |
| - |
| |
| - | Protocol 2: 5 mL Overnight
| |
| - |
| |
| - |
| |
| - | What you will need:
| |
| - |
| |
| - | * 10 mL culture tube Use 16mm x 160mm or 16mm x 125 mm
| |
| - | * 5 mL LB
| |
| - | * concentrated antibiotics
| |
| - | * Single colonies on a plate (Best not to start O/N from glycerol stocks)
| |
| - |
| |
| - |
| |
| - | Procedure:
| |
| - |
| |
| - |
| |
| - | * Pipet 1.5-20uL of the appropriate antibiotic to the appropriate working concentration into culture tube.
| |
| - | * Add 5 mL non-contaminated LB. Do this first, then add antibiotic
| |
| - | * Select single colony using a sterile instrument that has been cooled and inoculate the liquid culture.
| |
| - | * Place culture tube in incubator at 37 C overnight shaking vigorously (250 rpm).
| |
| - |
| |
| - | ==Notes==
| |
| - |
| |
| - | For I0500, culture overnight; then add 1 mM ITPG in morning and continue culture for another 3 hrs. Then miniprep.
| |
| - |
| |
| - | [[Team:Alberta/Notebook/protocols| Back]]
| |
| - | {{Team:Alberta/endMainContent}}
| |
Latest revision as of 17:16, 26 October 2010
"
