From 2010.igem.org
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- | Protocol 7: Gel Extraction
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- | What you will need:
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- | * Clean scalpel
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- | * Transilluminator
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- | * Eppendorf tube
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- | * Gel extraction kit
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- | * 55oC water bath
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- | * Vortex
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- | * Microfuge
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- | Procedure
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- | * Place the gel on the transilluminator. Put on face shield and gloves.
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- | * Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
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- | * Pry out the gel ‘cube’ and place it in an eppendorf tube.
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- | * Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
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- | [[Team:Alberta/Notebook/protocols| Back]]
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- | {{Team:Alberta/endMainContent}}
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Latest revision as of 17:15, 26 October 2010