Protocol/7

From 2010.igem.org

(Difference between revisions)
(New page: {{Team:Alberta/Head}} {{Team:Alberta/navbar|project=selected}} {{Team:Alberta/beginLeftSideBar}} {{Team:Alberta/endLeftSideBar}} {{Team:Alberta/beginRightSideBar}} {{Team:Alberta/endRig...)
(Removing all content from page)
 
(One intermediate revision not shown)
Line 1: Line 1:
-
{{Team:Alberta/Head}}
 
-
{{Team:Alberta/navbar|project=selected}}
 
-
 
-
{{Team:Alberta/beginLeftSideBar}}
 
-
{{Team:Alberta/endLeftSideBar}}
 
-
 
-
{{Team:Alberta/beginRightSideBar}}
 
-
{{Team:Alberta/endRightSideBar}}
 
-
 
-
{{Team:Alberta/beginMainContent}}
 
-
 
-
Protocol 7: Gel Extraction
 
-
 
-
What you will need:
 
-
 
-
* Clean scalpel
 
-
* Transilluminator
 
-
* Eppendorf tube
 
-
* Gel extraction kit
 
-
* 55oC water bath
 
-
* Vortex
 
-
* Microfuge
 
-
 
-
 
-
Procedure
 
-
 
-
* Place the gel on the transilluminator. Put on face shield and gloves.
 
-
* Turn on transilluminator and quickly make four slices; one on each side of the band you want to cut out and turn transilluminator off. Keep exposure to a minimum.
 
-
* Pry out the gel ‘cube’ and place it in an eppendorf tube.
 
-
* Follow instructions on pp. 25 of [http://www.qiagen.com/literature/render.aspx?id=103715 QIAQuick Spin Handbook].
 
-
 
-
[[Team:Alberta/Notebook/protocols| Back]]
 
-
{{Team:Alberta/endMainContent}}
 

Latest revision as of 17:15, 26 October 2010