From 2010.igem.org
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| - | {{Team:Alberta/beginMainContent}}
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| - | Protocol 8: Ligation
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| - | What you will need:
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| - | * Vector
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| - | * Insert
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| - | * Milli Q buffer
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| - | * 5X ligase buffer
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| - | * T4 DNA ligase
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| - | ** [http://tools.invitrogen.com/content/sfs/manuals/t4dnaligase_5U_man.pdf]
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| - | Volumes of vector and insert may vary but a typical 20 ul reaction might be
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| - | {|
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| - | | milliQ water || 11 ul
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| - | |-
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| - | | 5x ligase buffer || 4 ul
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| - | | vector (200 ng/ul) || 1 ul
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| - | | insert (200 ng/ul) || 3 ul
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| - | | ligase || 1 ul
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| - | |}
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| - | Incubate at R/T for 1 hrs or overnight at 12-16 C
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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| - | {{Team:Alberta/endMainContent}}
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Latest revision as of 17:15, 26 October 2010
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