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| - | Protocol 15: Making LB Plates
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| - | Makes 1L (~50 plates)
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| - | Notes:
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| - | *use AGAR not agarose!
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| - | *use an Erlenmeyer flask that is twice the volume of your solution (avoid spillage in autoclave)
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| - | *keep your flask on a stir plate with a stir rod
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| - | *put Aluminum foil on the top of the flask and put autoclave tape on
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| - | *DON`T let it solidify after autoclaving by keeping it on a stir plate while it cools.
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| - | *DON`T put tubes of antibiotics back into freezer.
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| - | 1.Add the following to 800 ml MilliQ H20
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| - | *10g Bacto-tryptone
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| - | *5g yeast extract
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| - | *10g NaCl
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| - | 2.Adjust pH to 7.5 with NaOH - optional
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| - | 3.Add 15g agar
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| - | 4.Melt agar into solution in the microwave - optional
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| - | 5.Adjust volume to 1L with MilliQ H20
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| - | 6.Autoclave on the liquid cycle (no drying time) (aka LIQ20) for 30 minutes.
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| - | 7.Wait for it to cool to touchable temperature and then add antibiotics if necessary. Amount of antibiotic required should be in the protocol binder.
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| - | 8.Pour plates, making sure they are color coded with the type of antibiotic that you used.
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| - | '''Making LB Broth'''
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| - | All of the above WITHOUT AGAR.
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| - | [[Team:Alberta/Notebook/protocols| Back]]
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