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	[[Media:Ligation_Protocol.pdf|Ligation]]		[[Media:Ligation_Protocol.pdf|Ligation]]
-	*Gingo Bioworks Protocol	+	*Ginko Bioworks Protocol
	*Jeremy Minty Protocol		*Jeremy Minty Protocol

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Revision as of 20:36, 6 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Ginko Bioworks Protocol
• Jeremy Minty Protocol