"

From 2010.igem.org

Revision as of 20:32, 6 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 20:34, 6 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
Line 15:		Line 15:
	'''Using Lab Equipment'''		'''Using Lab Equipment'''
-	[[Media:6-28-2010_Protocol_for_making_cultures_from_a_-80C_freezer_stock.pdf|Enigeering Research Building Autoclave]]	+	[[Media:7-6-2010_ERB_Autoclave_Liquid_Sterilization_Protocol.pdf|Enigeering Research Building Autoclave]]
	'''Cell Culture'''		'''Cell Culture'''

---

Revision as of 20:34, 6 July 2010

General Outline for Biobrick Manipulations

Protocols

Using Lab Equipment

Enigeering Research Building Autoclave

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

• Getting parts from the 360 well registry plates
• Competent cell preparation
• Electroporation

Miniprep

DNA Digest

Running a Gel

DNA Purification

Ligation

• Gingo Bioworks Protocol
• Jeremy Minty Protocol