Team:DTU-Denmark/Parts

From 2010.igem.org

(Difference between revisions)
Line 1: Line 1:
-
__NOTOC__
 
<html>
<html>
<img src="https://static.igem.org/mediawiki/2010/6/6b/Wiki_banner_967px.png"  align="center"> </img>
<img src="https://static.igem.org/mediawiki/2010/6/6b/Wiki_banner_967px.png"  align="center"> </img>
Line 147: Line 146:
   </tr>
   </tr>
</table>
</table>
-
 
+
<table cellpadding=10px>
 +
<tr>
 +
<td width="163px" height="100%" valign="top">
 +
  <font color="#990000" face="arial" size="3">
 +
<ul type="circle">
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols">Lab Protocols</a>
 +
<ul>
 +
<font size="2">
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#BioLector">BioLector</a></li>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#Ligations">Ligations</a></li>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#PCR_purification">PCR product purification</a></li>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#Plasmid_purifcation">Plasmid purification by miniprep</a></li>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#Restriction_Digestion">Restriction/Digestion</a></li>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Lab_protocols#Transformation ">Transformation </a></li>
 +
</font></ul>
 +
</li><br>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/BioLector">BioLector</a></li><br>
 +
<li><a href="https://2010.igem.org/Team:DTU-Denmark/Safety_protocols">Safety Protocols</a></li><br>
 +
</ul>
 +
</td>
 +
<td valign="top">
 +
<h1>BioBricks submitted</h1>
 +
<p align="justify">Divergent promoters from Gifsy1 phage and repressor
 +
Expression of any gene located upstream of this BioBrick will not be expressed. However, any gene located downstream will be expressed.
 +
Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli.
 +
Reference
 +
Registry ID: BBa_K374008 http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008
 +
</p>
 +
</td>
 +
<td width="163px" height="100%" valign="top">
 +
</td>
 +
</tr>
 +
  </font>
 +
</table>
<!-- Main content area -->
<!-- Main content area -->
Line 154: Line 186:
</html>
</html>
-
== ''' characterizing our Biobricks ''' ==
 
-
In order to help the community using our biobricks characterization and evaluation of the bricks should be done. (Also to get a better evaluation at the competition). We have typed all our biobricks into the registry base and collect information on the different parts there. Biofab, a synthetic biology company, have been working on developing a data sheet to describe biobricks, as electrical parts are beeing described. [http://www.biofab.org/first_datasheet Biofab datasheet description].
 
-
An example describing BBa_F2620 can be foud in the article by Drew Endy and colle [http://www.nature.com/nbt/journal/v26/n7/full/nbt1413.html standardization of synthetic biological parts] <br /> We have tried to use this standard and approach in our description of our genes.
 

Revision as of 16:36, 26 October 2010

Welcome to the DTU iGEM wiki!

Home The Team The Project Parts submitted Results Notebook Blog

BioBricks submitted

Divergent promoters from Gifsy1 phage and repressor Expression of any gene located upstream of this BioBrick will not be expressed. However, any gene located downstream will be expressed. Gifsy1 is a temperate phage present in many of the Salmonella enterica serovar Typhimurium strains. This part contains the Gifsy1 promoters and the Gifsy1 repressor. The promoters are divergent; consisting of pR[Gifsy1] and pRM[Gifsy1]. Downstream of the pRM[Gifsy1] promoter is the repressor gogR. gogR binds to the operator regions located between the two divergent promoters and is predicted to act both as an activator of the pRM[Gifsy1] and a repressor of the pR[Gifsy1]. The regulatory system is similar to the one found in lambda phages but induction is repressor cleavage independent. Instead small lexA regulated anti-repressor proteins bind to the repressor and prevents its DNA activity. The part has been used and tested in E. Coli. Reference Registry ID: BBa_K374008 http://partsregistry.org/wiki/index.php?title=Part:BBa_K374008


Parts

New for iGEM 2010 is the groupparts tag. This tag will generate a table with all of the parts that your team adds to your team sandbox. Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.

<groupparts>iGEM010 DTU-Denmark</groupparts>

Retrieved from "http://2010.igem.org/Team:DTU-Denmark/Parts"