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Revision as of 14:51, 26 October 2010 (view source) Mimmi (Talk | contribs) (→Gel) ← Older edit		Latest revision as of 14:52, 26 October 2010 (view source) Mimmi (Talk | contribs) (→Mimmi)
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	==== new colony PCR ====		==== new colony PCR ====

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Latest revision as of 14:52, 26 October 2010

Contents 1 Mimmi 1.1 clone non-CPP operon 1.1.1 colony PCR 1.1.2 Gel 1.1.3 new colony PCR 1.1.4 ON cultures

Mimmi

clone non-CPP operon

colony PCR

mix		x12		primers		samples
mastermix	24.5			pEX_VF		pEX.SOD.RBS.yCCS 1-4
DNA	0.5			pEX_VR		pEX.SOD.his.RBS.yCCS 1-4
tot	25µl					pEX.his.SOD.RBS.yCCS 1-4
						positive control (pSB1C3.LMWP.SOD.his)

Gel

well	sample	well	sample
1	ladder	12	ladder
2	pEX.S_yC 1	13	pEX.hS_yC 1
3	pEX.S_yC 2	14	pEX.hS_yC 2
4	pEX.S_yC 3	15	pEX.hS_yC 3
5	pEX.S_yC 4	16	pEX.hS_yC 4
6	pEX.Sh_yC 1
7	pC.Sh_yC 2
8	pC.Sh_yC 3
9	pC.Sh_yC 4

new colony PCR

mix		x7		primers		samples
mastermix	24.5			pEX_VF		pEX.SOD.his.RBS.yCCS 1-7
DNA	0.5			pEX_VR
tot	25µl

ON cultures

• pEX.SOD.RBS.yCCS 4
• pEX.his.SOD.RBS.yCCS 3
• pEX.SOD
• pEX.yCCS