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Revision as of 22:36, 5 July 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 22:37, 5 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
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	''autoclave to sterilize''		''autoclave to sterilize''
-	''add antibiotics when warm to touch (about 50C)''	+	''add antibiotics after autoclaving when warm to touch (about 50C)''
	''pour plates when warm to the touch (about 50C)''		''pour plates when warm to the touch (about 50C)''

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Revision as of 22:37, 5 July 2010

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics after autoclaving when warm to touch (about 50C)

pour plates when warm to the touch (about 50C)

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20C freezer