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Revision as of 02:48, 30 June 2010 (view source) Annlesn (Talk | contribs) ← Older edit		Revision as of 22:31, 5 July 2010 (view source) Annlesn (Talk | contribs) Newer edit →
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	*500 mL DI water		*500 mL DI water
	''autoclave to sterilize''		''autoclave to sterilize''
		+
		+	'''LB media with agar'''
		+	*10 g LB broth
		+	*7.5 g agar
		+	*500 mL DI water
		+	''autoclave to sterilize''
		+
		+	''add antibiotics when warm to touch (about 50C)''
		+
		+	''pour when warm to the touch (about 50C)''
	'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''		'''Kanamycin Antibiotic Stock Solution (50 mg/mL)'''

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Revision as of 22:31, 5 July 2010

Media Recipes

LB media

• 10 g LB broth
• 500 mL DI water

autoclave to sterilize

LB media with agar

• 10 g LB broth
• 7.5 g agar
• 500 mL DI water

autoclave to sterilize

add antibiotics when warm to touch (about 50C)

pour when warm to the touch (about 50C)

Kanamycin Antibiotic Stock Solution (50 mg/mL)

• 250 mg of kanamycin
• 5 mL of DI water

filter sterilize and make 1 mL aliquots to store in the -20C freezer

Tetracycline Antibiotic Stock Solution (50 mg/mL)

• 250 mg of tetracycline
• 2.5 mL of DI water
• 2.5 mL of ethanol

filter sterilize and make 1 mL aliquots to store in the -20C freezer