Team:Michigan/Protocols

From 2010.igem.org

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[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]
[[Media:General_DNA_Digest_Protocol.pdf|DNA Digest]]
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[[Media:General_DNA_Digest_Protocol.pdf|Running a Gel]]
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[[Media:Protocol_for_Running_a_Gel.pdf|Running a Gel]]

Revision as of 21:44, 5 July 2010


Michigan Header




Protocols

Cell Culture

Making cultures from a -80C freezer stock

Making frozen stocks

P. putida KT2440 antibotic resistance tolerance

DNA Manipulation

Transformation

  • Getting parts from the 360 well registry plates
  • Competent cell preparation
  • Electroporation

Miniprep

DNA Digest

Running a Gel