"
Team:ETHZ Basel/Achievements/BioBrick Toolbox
From 2010.igem.org
(Difference between revisions)
(→BioBrick Toolbox) |
(→BioBrick Toolbox) |
||
| Line 8: | Line 8: | ||
All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721) | All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721) | ||
| - | |||
<center> | <center> | ||
{| class="wikitable sortable" border="0" style="text-align: left" | {| class="wikitable sortable" border="0" style="text-align: left" | ||
Revision as of 11:37, 26 October 2010
BioBrick Toolbox
To construct the aimed fusion proteins, we first blunt-end cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721)
| ID | Insert | Orientation | ID | Insert | Orientation | Description |
| <partinfo>BBa_K422003</partinfo> | CheB | A-part | <partinfo>BBa_K422003</partinfo> | CheB | C-part | Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs |
| <partinfo>BBa_K422005</partinfo> | CheR | A-part | <partinfo>BBa_K422003</partinfo> | CheR | C-part | Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs |
| <partinfo>BBa_K422007</partinfo> | CheY | A-part | <partinfo>BBa_K422003</partinfo> | CheY | C-part | Induces tumbling by interacting with the flagellar switch protein FliM |
| <partinfo>BBa_K422009</partinfo> | tetR | A-part | Tetracyclin repressor: Binds to its operator tetO for spatial localization of a fusion protein to the DNA | |||
| <partinfo>BBa_K422010</partinfo> | trig | A-part | Ribosome binding domain of the trigger factor: Binds to the large subunit of the ribosome | |||
| <partinfo>BBa_K422011</partinfo> | MreB | C-part | Prokaryotic actin homologue: Assembles into helical filaments underneath the cytoplasmic membrane | |||
| <partinfo>BBa_K422012</partinfo> | Pif3 | C-part | Phytochrome interacting factor: Binds Prf form of PhyB and rapidly dissociates in response to reconversion to the Pr state | |||
| <partinfo>BBa_K422013</partinfo> | PhyB | C-part | Phytochromes type II: Biologically inactive form Pr absorbs red light and active configuration Pfr far-red light | |||
| <partinfo>BBa_K422014</partinfo> | mcyPet | A-part | CFP variant |
We also gave a lot of thoughts how to make BBF RFC28 (method for combinatorial multi-part assembly based on the Type II restriction enzyme AarI) compatible with Tom Knight's OAS standard. Check out our general scheme for the easy construction of Tom Knight's OAS and BBF RFC28 compatible working vectors [1]!
