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Team:Stockholm/26 July 2010
From 2010.igem.org
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== Mimmi == | == Mimmi == | ||
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| - | ==Sequencing CPP transportan 10 version C== | + | ===Sequencing CPP transportan 10 version C=== |
I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing. | I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing. | ||
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| - | ==Inoculation of IgG protease== | + | |
| + | ===Inoculation of IgG protease=== | ||
I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep. | I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep. | ||
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Latest revision as of 10:55, 26 October 2010
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Contents |
Mimmi
LMWP C and N
- Plasmid prep and glycerol stock
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
- DNA concentrations
Sample DNA (ng/μl) LMWP N 60 LMWP C 80
over-expression in pEX
- - remaking the comassie-gel
| Mix | |
|---|---|
| LB | 4ml |
| glycose | 40µl (1%) |
| Amp | 8µl (0.5%) |
- Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
Nina
Sequencing CPP transportan 10 version C
I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.
- 15 ul vector
- 1.5 ul 10uM VR2 primer
9: ASB003N X99
40: ASB003N X98
Inoculation of IgG protease
I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep.
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