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Team:Stockholm/26 July 2010
From 2010.igem.org
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| + | {{Stockholm/Top2}} | ||
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== Mimmi == | == Mimmi == | ||
=== LMWP C and N === | === LMWP C and N === | ||
| + | ::'''Plasmid prep and glycerol stock''' | ||
| + | |||
| - | |||
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid | The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid | ||
| - | + | ::'''DNA concentrations''' | |
| - | '''DNA concentrations''' | + | ::{|border="1" cellspacing="0" |
| - | {|border="1" cellspacing="0 | + | ! Sample |
| - | + | ! DNA (ng/μl) | |
| - | + | ||
|- | |- | ||
|LMWP N | |LMWP N | ||
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|} | |} | ||
| - | === over-expression in pEX - remaking the comassie-gel == | + | |
| - | + | ||
| - | + | === over-expression in pEX === | |
| - | + | ::'''- remaking the comassie-gel''' | |
| - | + | ||
| - | Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON | + | |
| + | {|border="1" cellspacing="0" | ||
| + | ! Mix | ||
| + | | | ||
| + | |- | ||
| + | | LB | ||
| + | | 4ml | ||
| + | |- | ||
| + | | glycose | ||
| + | | 40µl (1%) | ||
| + | |- | ||
| + | | Amp | ||
| + | | 8µl (0.5%) | ||
| + | |} | ||
| + | |||
| + | |||
| + | *Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ==Nina== | ||
| + | |||
| + | ===Sequencing CPP transportan 10 version C=== | ||
| + | |||
| + | I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing. | ||
| + | |||
| + | * 15 ul vector | ||
| + | |||
| + | * 1.5 ul 10uM VR2 primer | ||
| + | |||
| + | 9: ASB003N X99 | ||
| + | |||
| + | 40: ASB003N X98 | ||
| + | |||
| + | ---- | ||
| + | |||
| + | ===Inoculation of IgG protease=== | ||
| + | |||
| + | I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep. | ||
| + | |||
| + | {{Stockholm/Footer}} | ||
Latest revision as of 10:55, 26 October 2010
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Contents |
Mimmi
LMWP C and N
- Plasmid prep and glycerol stock
The cells were cultured to 0.6 OD, 1.6 ml were used to the glycerol stock and 10 ml were used to extract the plasmid
- DNA concentrations
Sample DNA (ng/μl) LMWP N 60 LMWP C 80
over-expression in pEX
- - remaking the comassie-gel
| Mix | |
|---|---|
| LB | 4ml |
| glycose | 40µl (1%) |
| Amp | 8µl (0.5%) |
- Add a pipette tip of BL21 pEX.BBa_J18932 from glycerol stock and grow ON
Nina
Sequencing CPP transportan 10 version C
I send two samples of CPP transportan 10 version C with colony number 9 and 40 for sequencing.
- 15 ul vector
- 1.5 ul 10uM VR2 primer
9: ASB003N X99
40: ASB003N X98
Inoculation of IgG protease
I placed the inoculation samples in the refrigerator for mini prep the following day when I know which sample is suitable for miniprep.
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