Team:Stockholm/8 June 2010

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(New page: {{Stockholm/Top2}} = Nina = === Preparation of competent DH5α ''Escherichia coli'' cells === ''Continued from 21/6''.<br /> Prepared competent DH5α cells according to the protocol of...)
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Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.
Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.
 +
 +
----
 +
 +
Absorbance:
 +
 +
* 1 ml sample from one bottle:
 +
 +
{|
 +
! Time
 +
!
 +
!
 +
!
 +
! OD
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,029
 +
|-
 +
|1h
 +
|
 +
|
 +
|
 +
|0,053
 +
|-
 +
|1h
 +
|
 +
|
 +
|
 +
|0,188
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,316
 +
 +
|}
 +
 +
*1 ml sample from two bottles:
 +
 +
{|
 +
! Time
 +
!
 +
!
 +
!
 +
! OD
 +
|-
 +
|30min
 +
|
 +
|
 +
|
 +
|0,519/0,503
 +
|-
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|7min
 +
|
 +
|
 +
|
 +
|0,578/0,566
 +
|-
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|5min
 +
|
 +
|
 +
|
 +
|0,598/0,600
 +
 +
 +
|}
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= Johan =
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== Continuation of making competent cells ==
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Step 2-12 in "800 ml of competent cells"
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1 L instead of 800 ml -> 25 st 50 ml Falcon Tubes with 40 ml, 1 st with 15-20 ml
 +
 +
10) 16/23 = 0,69 ml
 +
 +
3) Abs:
 +
 +
Only one bottle
 +
 +
* 0,029 9:25
 +
* 0,053 ~1h5min
 +
* 0,188 ~1h5min
 +
* 0,316 ~30min
 +
 +
Both bottles
 +
 +
* 0,519 ~30min
 +
* 0,503
 +
* 0,578 ~7min
 +
* 0,566
 +
* 0,598 ~5min = 13:05
 +
* 0,600
 +
 +
12) ~160 epps, 100 µl in each.
 +
 +
They were then all put in the freezer, in two cardboard boxes (no metal blocks used)
 +
 +
{{Stockholm/Footer}}

Latest revision as of 10:25, 26 October 2010

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Contents

Nina

Preparation of competent DH5α Escherichia coli cells

Continued from 21/6.

Prepared competent DH5α cells according to the protocol of Morten Nørholm at the Department of Biochemistry & Biophysics Stockholm University.

Absorbance:

  • 1 ml sample from one bottle:
Time OD
30min 0,029
1h 0,053
1h 0,188
30min 0,316
  • 1 ml sample from two bottles:
Time OD
30min 0,519/0,503
7min 0,578/0,566
5min 0,598/0,600


Johan

Continuation of making competent cells

Step 2-12 in "800 ml of competent cells"

1 L instead of 800 ml -> 25 st 50 ml Falcon Tubes with 40 ml, 1 st with 15-20 ml

10) 16/23 = 0,69 ml

3) Abs:

Only one bottle

  • 0,029 9:25
  • 0,053 ~1h5min
  • 0,188 ~1h5min
  • 0,316 ~30min

Both bottles

  • 0,519 ~30min
  • 0,503
  • 0,578 ~7min
  • 0,566
  • 0,598 ~5min = 13:05
  • 0,600

12) ~160 epps, 100 µl in each.

They were then all put in the freezer, in two cardboard boxes (no metal blocks used)





The Faculty of Science at Stockholm University Swedish Vitiligo association (Svenska Vitiligoförbundet) Geneious Fermentas/ Sigma-Aldrich/

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