Team:TU Delft/18 June 2010 content

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{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
{| style="color:black; background-color:white;" cellpadding="5" cellspacing="0" border="1"
|Sample  
|Sample  
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|Amount of SDS
+
|Amount of SDS (%)
-
|Hexalkane
+
|Hexalkane (mL)
|-
|-
|1
|1
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|-
|-
|2
|2
-
|0% SDS
+
|0
-
|0.02 mL
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|0.02
|-
|-
|3
|3
-
|0.01% SDS
+
|0.01
-
|0.02 mL
+
|0.02
|-
|-
|4
|4
-
|1% SDS
+
|1%  
-
|0.02 mL
+
|0.02
|}
|}
Tris buffer (pH 8) was added to a total volume of 7.5 ml.
Tris buffer (pH 8) was added to a total volume of 7.5 ml.
-
Incubate 1h at 30C at shaken at 200 RPM.
+
Incubate 1 hour at 30 °C at shaken at 200 RPM.
Absorbance measured at 600 nm.
Absorbance measured at 600 nm.

Revision as of 12:15, 5 July 2010

We had a nice slow day, finishing some small experiments and reorganizing our iGEM room. We hide all the stuff from last years team in a closet so now our room is nice and tidy.

We also booked a hotel in Boston!

Lab work

Pieter and Eva worked on the emulsifying activity assay. Four samples were tested:

Sample Amount of SDS (%) Hexalkane (mL)
1 Blank 0
2 0 0.02
3 0.01 0.02
4 1% 0.02

Tris buffer (pH 8) was added to a total volume of 7.5 ml.

Incubate 1 hour at 30 °C at shaken at 200 RPM.

Absorbance measured at 600 nm.

Sample A600
1 0
2 0
3 0.4
4 0.2
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