Team:Michigan/Pili Expression

From 2010.igem.org

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__NOTOC__
 
{{Michigan Header}}
{{Michigan Header}}
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{|style="color:#1c2bf2;background-color:#fafa19;font-size:9pt;text-align:center" cellpadding="5" cellspacing="0" border="1" bordercolor="#fff" width="62%"
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=Pili Expression Team=      
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!
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|Sunday
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|Monday
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|Tuesday
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|Wednesday
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|Thursday
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|Friday
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|Saturday
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!Week 1
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|[[Team:Michigan/Pili_Expression#6/28/2010|6/28/2010]]
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|[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]]
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|[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]]
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|[[Team:Michigan/Pili_Expression#7/1/2010|7/1/2010]]
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!Week 2
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==6/28/2010==
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This team includes [[User:MarcSinger|Marc Singer]], [[User:kevijose|Kevin Joseph]], and [[User:shanwu|Alena Wu]].
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Made a 500 mL batch of LB broth
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*When pouring in distilled water, pour a few mL at a time to avoid clumping.
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*It takes 20g of powder to make 1 L of broth
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Sterilized broth using the autoclave
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==Monthly Notebooks==
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*The temperature setting on the autoclave is off by a little bit
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In order to conserve page space, we have split our notebook according to months.
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*Set dial 2 notches below 134°C.
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==6/29/2010==
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[[Team:Michigan/Pili_June_July|June/July]]
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Started growing E. coli K12 cultures
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*Poured 2 mL of LB broth into a Falcon tube
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*Used strain of K12 from Dr. Lin's freezer
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*Placed in incubator shaker for 24 hrs.
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Added and inventoried supplies from Dr. Pinto's lab.
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[[Team:Michigan/Pili_August_September|August/September]]
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*Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
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*Chemical wastes must be cleaned by calling OSEH.
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==6/30/2010==
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[[Team:Michigan/Pili_October|October]]
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Cryopreserved stock of K12
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*1 mL located in the iGEM box in the Lin lab.
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*1 mL located in the lab freezer.
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==7/1/2010==
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=='''In the Lab'''==
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Cryopreserved DH5α according to protocol procedure on 6/30/2010
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<html>
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*Put 1 stock in -20°C fridge in 1239
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<iframe src="http://www-personal.umich.edu/~shanwu/wiki_photos/src/themes/classic/classic-demo_pili.html" width="100%" height="650px" scrolling="no"></iframe>
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*Put the other stock in the -80°C fridge in the Lin lab
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</html>

Latest revision as of 07:56, 26 October 2010


Michigan Header




Pili Expression Team

This team includes Marc Singer, Kevin Joseph, and Alena Wu.

Monthly Notebooks

In order to conserve page space, we have split our notebook according to months.

June/July

August/September

October

In the Lab

Retrieved from "http://2010.igem.org/Team:Michigan/Pili_Expression"