Team:Michigan/Pili Expression

From 2010.igem.org

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(New page: {{Michigan Header}} ==6/28/2010== Made a 500 mL batch of LB broth *When pouring in distilled water, pour a few mL at a time to avoid clumping. *It takes 20g of powder to make 1 L of broth...)
(In the Lab)
 
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{{Michigan Header}}
{{Michigan Header}}
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==6/28/2010==
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=Pili Expression Team=      
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Made a 500 mL batch of LB broth
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*When pouring in distilled water, pour a few mL at a time to avoid clumping.
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*It takes 20g of powder to make 1 L of broth
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Sterilized broth using the autoclave
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This team includes [[User:MarcSinger|Marc Singer]], [[User:kevijose|Kevin Joseph]], and [[User:shanwu|Alena Wu]].
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*The temperature setting on the autoclave is off by a little bit
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*Set dial 2 notches below 134 °C.
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==6/29/2010==
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==Monthly Notebooks==
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Started growing E. coli K12 cultures
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In order to conserve page space, we have split our notebook according to months.
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*Poured 2 mL of LB broth into a Falcon tube
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*Used strain of K12 from Dr. Lin's freezer
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*Placed in incubator shaker for 24 hrs.  
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Added and inventoried supplies from Dr. Pinto's lab.
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[[Team:Michigan/Pili_June_July|June/July]]
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*Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
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*Chemical wastes must be cleaned by calling OSEH.
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[[Team:Michigan/Pili_August_September|August/September]]
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[[Team:Michigan/Pili_October|October]]
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=='''In the Lab'''==
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<html>
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<iframe src="http://www-personal.umich.edu/~shanwu/wiki_photos/src/themes/classic/classic-demo_pili.html" width="100%" height="650px" scrolling="no"></iframe>
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</html>

Latest revision as of 07:56, 26 October 2010


Michigan Header




Pili Expression Team

This team includes Marc Singer, Kevin Joseph, and Alena Wu.

Monthly Notebooks

In order to conserve page space, we have split our notebook according to months.

June/July

August/September

October

In the Lab