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Minnesota-experimental/2 March 2010
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(New page: '''Rachel''' Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microlit...) |
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| + | |'''[[Minnesota-experimental/1 March 2010|Go to Previous Day (March 1)]]'''|| width=158|'''[[Minnesota-experimental/3 March 2010|Go to Next Day (March 3)]]''' | ||
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'''Rachel''' | '''Rachel''' | ||
Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction. | Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction. | ||
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'''Tony and Matt''' | '''Tony and Matt''' | ||
Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction). | Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction). | ||
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'''Rachel and Ian''' | '''Rachel and Ian''' | ||
Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight. | Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight. | ||
Latest revision as of 03:38, 26 October 2010
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Rachel Used PCR purification kit to purify digestion reaction. Performed ligation reaction with pucmod BB BglII/NotI vector and Eut MN BglII/NotI insert. Reaction was a 25 microliter reaction.
Tony and Matt Concentrated Eut LK solution in speed-vac for sixteen minutes. Eut LK solution digested with BglII/NotI (50 microliter reaction).
Rachel and Ian Transformation of 15 microliters of ligation reaction from 3/2. Plated on amp plates at 37 degree overnight.
