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Week of 10/4
From 2010.igem.org
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(New page: [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook]) |
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| + | ==October 5th== | ||
| + | |||
| + | *Digests: | ||
| + | -R1062+RFP (SP) | ||
| + | -R0061+RFP (SP) | ||
| + | |||
| + | *Ligations: | ||
| + | -R1062+RFP -->J23100+LuxR (B) | ||
| + | -->J23108+LuxR (B) | ||
| + | -->J23116+LuxR (B) | ||
| + | -R0063+RFP -->J23100+LuxR (B) | ||
| + | -->J23108+LuxR (B) | ||
| + | -->J23116+LuxR (B) | ||
| + | |||
| + | |||
| + | ==October 6th== | ||
| + | |||
| + | *Colony PCR: | ||
| + | 1. R1062+RFP + J23100+LuxR | ||
| + | 2. R1062+RFP + J23108+LuxR | ||
| + | 3. R1062+RFP + J23116+LuxR | ||
| + | 4. R0063+RFP + J23100+LuxR | ||
| + | 5. R0063+RFP + J23108+LuxR | ||
| + | 6. R0063+RFP + J23116+LuxR | ||
| + | |||
| + | They were then run on a gel after being PCRd. | ||
| + | |||
| + | *Gel Order: | ||
| + | |||
| + | |||
[https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | [https://2010.igem.org/Team:Penn_State/Notebook Return to Notebook] | ||
Revision as of 01:40, 26 October 2010
October 5th
- Digests:
-R1062+RFP (SP) -R0061+RFP (SP)
- Ligations:
-R1062+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
-R0063+RFP -->J23100+LuxR (B)
-->J23108+LuxR (B)
-->J23116+LuxR (B)
October 6th
- Colony PCR:
1. R1062+RFP + J23100+LuxR 2. R1062+RFP + J23108+LuxR 3. R1062+RFP + J23116+LuxR 4. R0063+RFP + J23100+LuxR 5. R0063+RFP + J23108+LuxR 6. R0063+RFP + J23116+LuxR
They were then run on a gel after being PCRd.
- Gel Order:
