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Team:Panama/13 August 2010
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Revision as of 23:59, 25 October 2010
August 13
Digestion Reaction
Now that we have our Rh1ab we are going to run a PCR of it using our first set of primers.
RhT-F1a 5' GCGATAGCTGTTTGCCTGTT 3'
RhT-R2a 5' TGGCAACCCTATCTGTTATGC 3'
First of all, we have to resuspend our primers since they come in a lyophilized manner. We need a stock solution of 100uM. Our working solution has a concentration of 10uM.
We tested three concentrations of primers to see which one worked better for us and we can see that all three concentrations worked! So from now on, just to be on the safe side, we're going to use the concentration of 1uM.
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