Team:UNIPV-Pavia/Material Methods/Measurements/Tecan/test1settembre

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Line 29: Line 29:
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
-
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300012</partinfo> (wiki name: I12)
+
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
* <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
Line 40: Line 40:
* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
* <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)
-
==RESULTS (?)==
+
==RESULTS==
 +
Data were analyzed following the [[Team:UNIPV-Pavia/Parts/Characterization#Data analysis for self-inducible promoters (initiation-treshold determination)| data analysis procedure]] to evaluate the ODstart. The plots correpsoning to the parts tested are reported:
 +
[[Image:UNIPV_Pavia_FluoVSODHC0109.png| 620px | center ]]
 +
 +
[[Image:UNIPV_Pavia_FluoVSODCo0109.png| 620px | center ]]
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 +
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The ODstart are reported in the table below:
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{| border='1' align='center'
 +
|| Part || ODstart
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> || 0.0234
 +
|-
 +
|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> || -
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> || 0.3366
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> || 0.2762
 +
|-
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|| <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> || 0.2452
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> || 0.0425
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> || 0.1420
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> || -
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> || 0.0765
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> || -
 +
|-
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|| <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> || 0.2430
 +
 +
|}
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Latest revision as of 21:23, 25 October 2010
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SEPTEMBER, 1ST


PLATE


UNIPV Pavia piastra1settembre.png


EXPERIMENT DESCRIPTION

Motivation

The experiment was performed to evaluate the initiation transcription point (in term of absorbance) for self-inducible devices.

Methods

The experiment was performed in M9 according to Protocol 1 on the following measurement parts:

  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> (wiki name: I7)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> (wiki name: I8)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> (wiki name: I9)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> (wiki name: I10)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> (wiki name: I12)
  • <partinfo>pSB1A2</partinfo> - <partinfo>K173001</partinfo> (positive control)
  • <partinfo>pSB1A2</partinfo> - <partinfo>BBa_B0032</partinfo> (negative control)
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> (wiki name I14) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> (wiki name I15) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> (wiki name I16) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> (wiki name I17) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> (wiki name I18) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> (wiki name I19) co-transformed with <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo>
  • <partinfo>pSB1A3</partinfo> - <partinfo>BBa_T9002</partinfo> (negative control)

RESULTS

Data were analyzed following the data analysis procedure to evaluate the ODstart. The plots correpsoning to the parts tested are reported:

UNIPV Pavia FluoVSODHC0109.png
UNIPV Pavia FluoVSODCo0109.png


The ODstart are reported in the table below:

Part ODstart
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300024</partinfo> 0.0234
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300021</partinfo> -
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300022</partinfo> 0.3366
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300023</partinfo> 0.2762
<partinfo>pSB1A2</partinfo> - <partinfo>BBa_K300019</partinfo> 0.2452
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300030</partinfo> 0.0425
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300028</partinfo> 0.1420
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300029</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300025</partinfo> 0.0765
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300026</partinfo> -
<partinfo>pSB4C5</partinfo> - <partinfo>BBa_K300027</partinfo> 0.2430



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