"
Team:UNIPV-Pavia/Calendar/June
From 2010.igem.org
(Difference between revisions)
m |
|||
| Line 16: | Line 16: | ||
<tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr> | <tr><td align="center" valign="top"><html><a name="June_3rd"></a></html>June, 3rd</td><td align="left">9° Meeting - Discussion of final and technical details about [[Team:UNIPV-Pavia/Project|The Project]]. Planning of Bio-Lab activity.</td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 7th|June, 7th]]</td><td align="left"><partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in ''E. coli'' DH5alpha.<br>Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".</td></tr> | ||
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 8th|June, 8th]]</td><td align="left">Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep.<br></td></tr> |
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 9th|June, 9th]]</td><td align="left">MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 9th|June, 9th]]</td><td align="left">MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: | ||
*I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) | *I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) | ||
| Line 22: | Line 22: | ||
*I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) | *I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) | ||
*I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)<br></td></tr> | *I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)<br></td></tr> | ||
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 10th|June, 10th]]</td><td align="left">Transformation of ligations I0, I1, I2, I3 in home made ''E. coli'' DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 10th|June, 10th]]</td><td align="left">Transformation of ligations I0, I1, I2, I3 in home made ''E. coli'' DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates.<br></td></tr> |
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 11th|June, 11th]]</td><td align="left">Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).<br></td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 11th|June, 11th]]</td><td align="left">Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).<br></td></tr> | ||
<tr><td align="center" valign="top"><html><a name="June_12th"></a></html>June, 12th</td><td align="left">iGEM EU workshop<br></td></tr> | <tr><td align="center" valign="top"><html><a name="June_12th"></a></html>June, 12th</td><td align="left">iGEM EU workshop<br></td></tr> | ||
| Line 32: | Line 32: | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 18th|June, 18th]]</td><td align="left">10° Bio-Lab - Susanna Z., Susanna S. and Federica.<br></td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 18th|June, 18th]]</td><td align="left">10° Bio-Lab - Susanna Z., Susanna S. and Federica.<br></td></tr> | ||
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 21st|June, 21st]]</td><td align="left">11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.<br></td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 21st|June, 21st]]</td><td align="left">11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.<br></td></tr> | ||
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 22nd|June, 22nd]]</td><td align="left">12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 22nd|June, 22nd]]</td><td align="left">12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò.<br></td></tr> |
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 23rd|June, 23rd]]</td><td align="left">13° Bio-Lab - Sara, Susanna Z., Nicolò.<br>10° Meeting - Updating about Bio-Lab activity</td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 23rd|June, 23rd]]</td><td align="left">13° Bio-Lab - Sara, Susanna Z., Nicolò.<br>10° Meeting - Updating about Bio-Lab activity.</td></tr> |
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 24th|June, 24th]]</td><td align="left">14° Bio-Lab - Sara, Susanna Z., Alessandro.<br></td></tr> | ||
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana4 #June, 25th|June, 25th]]</td><td align="left">15° Bio-Lab - Susanna Z., Chiara, Riccardo.<br></td></tr> |
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 28th|June, 28th]]</td><td align="left">16° Bio-Lab - Susanna Z., Nicolò, Federica, Giacomo.<br></td></tr> |
| - | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo<br></td></tr> | + | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 29th|June, 29th]]</td><td align="left">17° Bio-Lab - Susanna Z., Nicolò, Riccardo,Giacomo, Lorenzo.<br></td></tr> |
<tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 30th|June, 30th]]</td><td align="left">18° Bio-Lab - Susanna Z.<br></td></tr> | <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/July/settimana1 #June, 30th|June, 30th]]</td><td align="left">18° Bio-Lab - Susanna Z.<br></td></tr> | ||
</table> | </table> | ||
Revision as of 13:05, 2 July 2010
|
|
|||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
