Team:Newcastle/14 July 2010

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(New page: Performed a gel electrophoresis with remaining PCR products + Controls with same restriction digests. Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday) ...)
(Research)
 
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Performed a gel electrophoresis  with remaining PCR products + Controls with same restriction digests.
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{{Team:Newcastle/mainbanner}}
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Aim: to see whether the LacI insert is in the pSB1AT3 plasmid (same as yesterday)
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=Research=
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Protocol: we followed Wendy's cards/Phil's lab-book
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We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.
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#Kleerebezem M, Bongers R, Rutten G, Vos WM de and Kuipers OP. (2004). "Autoregulation of subtilin biosynthesis in Bacillus subtilis: the role of the spa-box in subtilin-responsive promoters". ''Peptides''. 25(9). 1415-24.
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#Klein C, Kaletta C, Schnell N and Entian KD. (1992). "Analysis of genes involved in biosynthesis of the lantibiotic subtilin". ''Applied and environmental microbiology''. 58(1). 132-42.
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{{Team:Newcastle/footer}}

Latest revision as of 20:15, 25 October 2010

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Research

We initiated our research on subtilin quorum sensing system which would provide a coordinated cell response.

  1. Kleerebezem M, Bongers R, Rutten G, Vos WM de and Kuipers OP. (2004). "Autoregulation of subtilin biosynthesis in Bacillus subtilis: the role of the spa-box in subtilin-responsive promoters". Peptides. 25(9). 1415-24.
  2. Klein C, Kaletta C, Schnell N and Entian KD. (1992). "Analysis of genes involved in biosynthesis of the lantibiotic subtilin". Applied and environmental microbiology. 58(1). 132-42.
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