Team:UNIPV-Pavia/Calendar/June

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Revision as of 08:37, 2 July 2010

JUNE

DATE	ACTIVITY
June, 3th	9° Meeting - Discussion of final and technical details about The Project. Planning of Bio-Lab activity.
June, 7th	<partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo> were resuspended from Spring 2010 DNA distribution. <partinfo>BBa_P1004</partinfo> was resuspended from Spring 2009 DNA distribution. All BioBricks were transformed in E. coli DH5alpha. Liquid LB+Amp and LB+Amp agar plates were prepared according with "protocols".
June, 8th	Inoculum from plates in liquid Lb and glycerol stocks preparation. Falcon were re-filled for tomorrow mini-prep
June, 9th	MiniPrep of <partinfo>BBa_E2050</partinfo>, <partinfo>BBa_K165018</partinfo>, <partinfo>BBa_K165037</partinfo>, <partinfo>BBa_J61001</partinfo>, <partinfo>BBa_K081008</partinfo> and <partinfo>BBa_K125500</partinfo>. Digestion, gel run/cut and ligation of: I0: <partinfo>BBa_K125500</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I1: <partinfo>BBa_E2050</partinfo> (E-S)+<partinfo>BBa_K165018</partinfo> (E-X) I2: <partinfo>BBa_P1004</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X) I3: <partinfo>BBa_K081008</partinfo> (E-S)+<partinfo>BBa_B0015</partinfo> (E-X)
June, 10th	Transformation of ligations I0, I1, I2, I3 in home made E. coli DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates
June, 11th	Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).
<a name="June, 12th"/>June, 12th	iGEM EU workshop
<a name="June, 13th"/>June, 13th	iGEM EU workshop
June, 14th	6° Bio-Lab - Lorenzo and Nicolò.
June, 15th	7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro.
June, 16th	8° Bio-Lab - Susanna Z., Sara and Nicolò.
June, 17th	9° Bio-Lab - Susanna Z., Nicolò and Riccardo.
June, 18th	10° Bio-Lab - Susanna Z., Susanna S. and Federica.
June, 21th	11° Bio-Lab - Nicolò, Manuel Z., Susanna Z.
June, 22th	12° Bio-Lab - Susanna Z., Lorenzo, Sara, Nicolò
June, 23th	13° Bio-Lab - Sara, Susanna Z., Nicolò.
June, 24th	14° Bio-Lab - Sara, Susanna Z., Alessandro.
June, 25th	15° Bio-Lab - Susanna Z., Chiara, Riccardo

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 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 10th|June, 10th]]</td><td align="left">Transformation of ligations I0, I1, I2, I3 in home made ''E. coli'' DH5-alpha competent cells. Transformants were plated on LB+Amp agar plates<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana2 #June, 11th|June, 11th]]</td><td align="left">Inoculum from plates in liquid LB and glycerol stocks preparation for I0, I1, I2 and I3 in duplicate (2 colonies peaked from each plate).<br></td></tr>
-<tr><td align="center" valign="top">June, 12th</td><td align="left">iGEM EU workshop<br></td></tr>
+<tr><td align="center" valign="top"><a name="June, 12th"/>June, 12th</td><td align="left">iGEM EU workshop<br></td></tr>
-<tr><td align="center" valign="top">June, 13th</td><td align="left">iGEM EU workshop<br></td></tr>
+<tr><td align="center" valign="top"><a name="June, 13th"/>June, 13th</td><td align="left">iGEM EU workshop<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 14th|June, 14th]]</td><td align="left">6° Bio-Lab - Lorenzo and Nicolò.<br></td></tr>
 <tr><td align="center" valign="top">[[Team:UNIPV-Pavia/Calendar/June/settimana3 #June, 15th|June, 15th]]</td><td align="left">7° Bio-Lab - Lorenzo and Manuel L., Sara and Alessandro.<br></td></tr>