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		+	== Expression of SOD and yCCS from pEX expression vector ==
		+	Two cell cultures of DH5alpha cells respectively transformed with pEX vectors carrying superoxidase dismutase (SOD) and yeast copper chaperone (yCCS) were previously prepared and received from Nina and Johan.
		+
		+	=====Procedures=====
		+	* An ON starter culture was set by inoculating 5 ml LB + 100 ug/ml Amp with 5 ul of each culture.
		+	** 37°C, 250 rpm.
		+
		+	''Continued 30/6''

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Revision as of 19:00, 1 July 2010

Andreas

Expression of SOD and yCCS from pEX expression vector

Two cell cultures of DH5alpha cells respectively transformed with pEX vectors carrying superoxidase dismutase (SOD) and yeast copper chaperone (yCCS) were previously prepared and received from Nina and Johan.

Procedures

• An ON starter culture was set by inoculating 5 ml LB + 100 ug/ml Amp with 5 ul of each culture.
  • 37°C, 250 rpm.

Continued 30/6