Team:ETHZ Basel/Achievements/BioBrick Toolbox

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| Pif3
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| Phytochrome interacting factor: N-Terminus of Pif3 selectively binds the Prf form of the phytochrome and rapidly dissociates in response to reconversion to the Pr state.  
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| Phytochrome interacting factor: Binds Prf form of PhyB and rapidly dissociates in response to reconversion to the Pr state.  
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| PhyB
| PhyB
| C-part
| C-part
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| Phytochromes type II: Exists in an biologically inactive form Pr absorbing red light and an active configuration Pfr which absorbs far-red light.
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| Phytochromes type II: Biologically inactive form Pr absorbs red light and active configuration Pfr far-red light.
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Revision as of 14:32, 25 October 2010

BioBrick Toolbox

BioBrick assembly A-parts are used for N-Terminal and C-parts for C-terminal fusion. B-part is the linker that connects the two entities.

To construct the aimed fusion proteins, we first blunt-end cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721).

Check out our general scheme for the easy construction of Tom Knight's OAS and BBF RFC28 compatible working vectors [1]!




Chemotaxis BrickBox

Name Insert Orientation Name Insert Orientation Description Favourite part
<partinfo>BBa_K422003</partinfo> CheB A-part <partinfo>BBa_K422004</partinfo> CheB C-part Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422005</partinfo> CheR A-part <partinfo>BBa_K422006</partinfo> CheR C-part Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422007</partinfo> CheY A-part <partinfo>BBa_K422008</partinfo> CheY C-part Induces tumbling by interacting with the flagellar switch protein FliM


Anchor BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422009</partinfo> TetR A-part Tetracyclin repressor: Binds to its operator tetO for spatial localization of a fusion protein to the DNA.
<partinfo>BBa_K422010</partinfo> trigA A-part Ribosome binding domain of the trigger factor: Binds to the large subunit of the ribosome.
<partinfo>BBa_K422011</partinfo> MreB C-part Prokaryotic actin homologue: Assembles into helical filaments underneath the cytoplasmic membrane.


Ligth sensor BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422012</partinfo> Pif3 C-part Phytochrome interacting factor: Binds Prf form of PhyB and rapidly dissociates in response to reconversion to the Pr state.
<partinfo>BBa_K422013</partinfo> PhyB C-part Phytochromes type II: Biologically inactive form Pr absorbs red light and active configuration Pfr far-red light.


Reporter BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422014</partinfo> cyPet A-part CFP variant.
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