Team:ETHZ Basel/Achievements/BioBrick Toolbox

From 2010.igem.org

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A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.
A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.
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== Chemotaxis BrixBox ==
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== Chemotaxis BrickBox ==
{| border="1"
{| border="1"
|-
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|}
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== Anchor BrixBox ==
 
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== Ligth sensor BrixBox ==
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== Anchor BrickBox ==
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{| border="1"
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|-
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! Name
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! Insert
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! Orientation
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! Description
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! Favourite part
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|-
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| <partinfo>BBa_K422009</partinfo>
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| TetR
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| A-part
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| Tetracyclin repressor: Binds to its operator tetO for spatial localization of a fusion protein to the DNA.
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|
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|-
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| <partinfo>BBa_K422010</partinfo>
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| trigA
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| A-part
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| Ribosome binding domain of the trigger factor: Binds to the large subunit of the ribosome.
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|
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|-
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| <partinfo>BBa_K422011</partinfo>
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| MreB
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| C-part
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| Prokaryotic actin homologue: Assembles into helical filaments underneath the cytoplasmic membrane.
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|
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|}
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== Ligth sensor BrickBox ==
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{| border="1"
 +
|-
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! Name
 +
! Insert
 +
! Orientation
 +
! Description
 +
! Favourite part
 +
|-
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| <partinfo>BBa_K422012</partinfo>
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| Pif3
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| C-part
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| Phytochrome interacting factor: N-Terminus of Pif3 selectively binds the Prf form of the phytochrome and rapidly dissociates in response to reconversion to the Pr state.
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|
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|-
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| <partinfo>BBa_K422013</partinfo>
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| PhyB
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| C-part
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| Phytochromes type II: Exists in a biologically inactive form Pr absorbing red light and an active configuration Pfr which absorbs far-red light.
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|
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|}
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== Reporter BrickBox ==
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{| border="1"
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|-
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! Name
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! Insert
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! Orientation
 +
! Description
 +
! Favourite part
 +
|-
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| <partinfo>BBa_K422014</partinfo>
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| cyPet
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| A-part
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| CFP variant.
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|
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|}

Revision as of 14:08, 25 October 2010

BioBrick Toolbox

To construct the aimed fusion proteins, we first cloned our BioBricks into the storage vector pSEVA132 <partinfo>BBa_K422002</partinfo> (Victor de Lorenzo's lab, KanR, pBBR1 ori). All parts are sequence verified and compatible with BBF RFC28 (http://dspace.mit.edu/handle/1721.1/46721). A-parts N-Terminal fusion and C-parts C-terminal fusion. B-part is the linker that connects the two entities.

Chemotaxis BrickBox

Name Insert Orientation Name Insert Orientation Description Favourite part
<partinfo>BBa_K422003</partinfo> CheB A-part <partinfo>BBa_K422004</partinfo> CheB C-part Demthylase: Demethylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422005</partinfo> CheR A-part <partinfo>BBa_K422006</partinfo> CheR C-part Methyltransferase: Methylates methyl accepting chemotaxis proteins MCPs
<partinfo>BBa_K422007</partinfo> CheY A-part <partinfo>BBa_K422008</partinfo> CheY C-part Induces tumbling by interacting with the flagellar switch protein FliM


Anchor BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422009</partinfo> TetR A-part Tetracyclin repressor: Binds to its operator tetO for spatial localization of a fusion protein to the DNA.
<partinfo>BBa_K422010</partinfo> trigA A-part Ribosome binding domain of the trigger factor: Binds to the large subunit of the ribosome.
<partinfo>BBa_K422011</partinfo> MreB C-part Prokaryotic actin homologue: Assembles into helical filaments underneath the cytoplasmic membrane.


Ligth sensor BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422012</partinfo> Pif3 C-part Phytochrome interacting factor: N-Terminus of Pif3 selectively binds the Prf form of the phytochrome and rapidly dissociates in response to reconversion to the Pr state.
<partinfo>BBa_K422013</partinfo> PhyB C-part Phytochromes type II: Exists in a biologically inactive form Pr absorbing red light and an active configuration Pfr which absorbs far-red light.


Reporter BrickBox

Name Insert Orientation Description Favourite part
<partinfo>BBa_K422014</partinfo> cyPet A-part CFP variant.
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