Team:LMU-Munich/Schedule

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==PCR and mutagenisis of the digestion sites==
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==PCR and mutagenesis of the digestion sites==
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<b>[[Primer table|Primer table]] : Primer sequences, intern numeration, Tm</b>
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<b>[[Team:LMU-Munich/Schedule/Primer table|Primer table]] : Primer sequences, intern numeration, Tm</b>
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Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
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==In Vitro testing of the chemical kinetics with the enzymes==
 
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==Transforming the construct in bacteria==
 
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==Testing the system==
 
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Latest revision as of 13:59, 25 October 2010


PCR and mutagenesis of the digestion sites

Primer table : Primer sequences, intern numeration, Tm


PCR1a: replication of Pdu D with mutagenesis [ ] PCR1aP.jpg

Primer used: 1P1D, mpduD rev expected product size: bp


PCR1b: replication of Pdu D with mutagenesis [ ] PCR1bP.jpg

Primer used: mpduD fwd, 3P2D expected product size: bp


PCR2: Joining PCR of PduD [ ] PCR2P.jpg

Primer used: 1P1D, 3P2D expected product size: bp


PCR3a: replication of Pdu C with mutagenesis [ ] PCR3aP.jpg

Primer used: 2P1C, mpduC rev expected product size: bp


PCR3b: replication of Pdu C with mutagenesis [ ] PCR3bP.jpg

Primer used: mpduC fwd, 4P2C expected product size: bp


PCR4: Joining PCR of Pdu C [ ] PCR4P.jpg

Primer used: 2P1C, 4P2C expected product size: bp


PCR5: replication of AurF to be joined with Pdu D [ ] PCR5P.jpg

Primer used: 5P3AD, 9P4A expected product size: bp


PCR6a: replication of LctO with mutagenesis [ ] PCR6aP.jpg

Primer used: 8P3LC, m1lctO rev expected product size: bp


PCR6b: replication of LctO with mutagenesis [ ] PCR6bP.jpg

Primer used: m1lctO fwd, 10P4L expected product size: bp


PCR7: Joining PCR of LctO [ ] PCR7P.jpg

Primer used: 8P3LC, 10P4L expected product size: bp


PCR8a: replication of LctO with mutagenesis [ ] PCR8aP.jpg

Primer used: 8P3LC, m2lctO rev expected product size: bp


PCR8b: replication of LctO with mutagenesis [ ] PCR8bP.jpg

Primer used: m2lctO fwd, 10P4L expected product size: bp


PCR9: Joining PCR of LctO [ ] PCR9P.jpg

Primer used: 8P3LC, 10P4L expected product size: bp


PCR10: Joining PCR of AurF and PduD [ ] PCR10P.jpg

Primer used: 1P1D, 9P4A expected product size: bp


PCR11: Joining PCR of LctO and PduC [ ] PCR11P.jpg

Primer used: 2P1C, 10P4L expected product size: bp


PCR12a: replication of the Pdu-Operon with mutagenesis [ ] PCR12aP.jpg

Primer used: pduA fwd, mpduA rev expected product size: bp


PCR12b: replication of the Pdu-Operon with mutagenesis [ ] PCR12bP.jpg


Primer used: mpduA fwd,pduU rev expected product size: bp


PCR13: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used: pduA fwd,pduU rev expected product size: bp


PCR14a: replication of the Pdu-Operon with mutagenesis [ ] PCR14aP.jpg

Primer used: pduA fwd,mpduB rev expected product size: bp


PCR14b: replication of the Pdu-Operon with mutagenesis [ ] PCR14bP.jpg

Primer used: mpduB fwd,pduU rev expected product size: bp


PCR15: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:pduA fwd,pduU rev expected product size: bp


PCR16a: replication of the Pdu-Operon with mutagenesis [ ] PCR16aP.jpg

Primer used:pduA fwd,mpduJ rev expected product size: bp


PCR16b: replication of the Pdu-Operon with mutagenesis [ ] PCR16bP.jpg

Primer used:mpduJ fwd,pduU rev expected product size: bp


PCR17: Joining PCR of the Pdu-Operon [ ] PCR131517P.jpg

Primer used:pduA fwd,pduU rev expected product size: bp

 

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