Team:LMU-Munich/Schedule

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Latest revision as of 13:59, 25 October 2010


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PCR and mutagenesis of the digestion sites

Primer table : Primer sequences, intern numeration, Tm

PCR1a: replication of Pdu D with mutagenesis [ ]

Primer used: 1P1D, mpduD rev expected product size: bp

PCR1b: replication of Pdu D with mutagenesis [ ]

Primer used: mpduD fwd, 3P2D expected product size: bp

PCR2: Joining PCR of PduD [ ]

Primer used: 1P1D, 3P2D expected product size: bp

PCR3a: replication of Pdu C with mutagenesis [ ]

Primer used: 2P1C, mpduC rev expected product size: bp

PCR3b: replication of Pdu C with mutagenesis [ ]

Primer used: mpduC fwd, 4P2C expected product size: bp

PCR4: Joining PCR of Pdu C [ ]

Primer used: 2P1C, 4P2C expected product size: bp

PCR5: replication of AurF to be joined with Pdu D [ ]

Primer used: 5P3AD, 9P4A expected product size: bp

PCR6a: replication of LctO with mutagenesis [ ]

Primer used: 8P3LC, m1lctO rev expected product size: bp

PCR6b: replication of LctO with mutagenesis [ ]

Primer used: m1lctO fwd, 10P4L expected product size: bp

PCR7: Joining PCR of LctO [ ]

Primer used: 8P3LC, 10P4L expected product size: bp

PCR8a: replication of LctO with mutagenesis [ ]

Primer used: 8P3LC, m2lctO rev expected product size: bp

PCR8b: replication of LctO with mutagenesis [ ]

Primer used: m2lctO fwd, 10P4L expected product size: bp

PCR9: Joining PCR of LctO [ ]

Primer used: 8P3LC, 10P4L expected product size: bp

PCR10: Joining PCR of AurF and PduD [ ]

Primer used: 1P1D, 9P4A expected product size: bp

PCR11: Joining PCR of LctO and PduC [ ]

Primer used: 2P1C, 10P4L expected product size: bp

PCR12a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: pduA fwd, mpduA rev expected product size: bp

PCR12b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: mpduA fwd,pduU rev expected product size: bp

PCR13: Joining PCR of the Pdu-Operon [ ]

Primer used: pduA fwd,pduU rev expected product size: bp

PCR14a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: pduA fwd,mpduB rev expected product size: bp

PCR14b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used: mpduB fwd,pduU rev expected product size: bp

PCR15: Joining PCR of the Pdu-Operon [ ]

Primer used:pduA fwd,pduU rev expected product size: bp

PCR16a: replication of the Pdu-Operon with mutagenesis [ ]

Primer used:pduA fwd,mpduJ rev expected product size: bp

PCR16b: replication of the Pdu-Operon with mutagenesis [ ]

Primer used:mpduJ fwd,pduU rev expected product size: bp

PCR17: Joining PCR of the Pdu-Operon [ ]

Primer used:pduA fwd,pduU rev expected product size: bp

@@ Line 1: / Line 1: @@
 {{:Team:LMU-Munich/Templates/Page Header}}
-==PCR and mutagenisis of the digestion sites==
+==PCR and mutagenesis of the digestion sites==
+<b>[[Team:LMU-Munich/Schedule/Primer table|Primer table]] : Primer sequences, intern numeration, Tm</b>
 <b>PCR1a: replication of Pdu D with mutagenesis [ ] </b>
 [[Image:PCR1aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/mpduD_rev| mpduD_rev]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/mpduD_rev| mpduD rev]] expected product size:  bp
@@ Line 11: / Line 17: @@
 [[Image:PCR1bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/mpduD_fwd| mpduD fwd]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
@@ Line 17: / Line 23: @@
 [[Image:PCR2P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/3P2D| 3P2D]] expected product size:  bp
@@ Line 23: / Line 29: @@
 [[Image:PCR3aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/mpduC_rev| mpduC rev]] expected product size:  bp
@@ Line 29: / Line 35: @@
 [[Image:PCR3bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/mpduC_fwd| mpduC fwd]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
@@ Line 35: / Line 41: @@
 [[Image:PCR4P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/4P2C| 4P2C]] expected product size:  bp
@@ Line 41: / Line 47: @@
 [[Image:PCR5P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/5P3AD| 5P3AD]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
@@ Line 47: / Line 53: @@
 [[Image:PCR6aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m1lctO_rev| m1lctO rev]] expected product size:  bp
@@ Line 53: / Line 59: @@
 [[Image:PCR6bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/m1lctO_fwd| m1lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
@@ Line 59: / Line 65: @@
 [[Image: PCR7P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
@@ Line 65: / Line 71: @@
 [[Image:PCR8aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/m2lctO_rev| m2lctO rev]] expected product size:  bp
@@ Line 71: / Line 77: @@
 [[Image:PCR8bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/m2lctO_fwd| m2lctO fwd]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
@@ Line 77: / Line 83: @@
 [[Image:PCR9P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/8P3LC| 8P3LC]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
@@ Line 83: / Line 89: @@
 [[Image:PCR10P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]][[Team:LMU-Munich/Schedule/9P4A| 9P4A]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/1P1D| 1P1D]],[[Team:LMU-Munich/Schedule/9P4A| 9P4A]] expected product size:  bp
@@ Line 89: / Line 95: @@
 [[Image:PCR11P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]][[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/2P1C| 2P1C]],[[Team:LMU-Munich/Schedule/10P4L| 10P4L]] expected product size:  bp
@@ Line 95: / Line 101: @@
 [[Image:PCR12aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduA_rev| mpduA rev]]  expected product size:  bp
@@ Line 102: / Line 108: @@
-Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/mpduA_fwd| mpduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
@@ Line 108: / Line 114: @@
 [[Image:PCR131517P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
@@ Line 114: / Line 120: @@
 [[Image:PCR14aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]][[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd| pduA fwd]],[[Team:LMU-Munich/Schedule/mpduB_rev|mpduB rev]] expected product size:  bp
@@ Line 120: / Line 126: @@
 [[Image:PCR14bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/mpduB_fwd| mpduB fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
@@ Line 126: / Line 132: @@
 [[Image:PCR131517P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
@@ Line 132: / Line 138: @@
 [[Image:PCR16aP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/mpduJ_rev|mpduJ rev]] expected product size:  bp
@@ Line 138: / Line 144: @@
 [[Image:PCR16bP.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/mpduJ_fwd|mpduJ fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
@@ Line 144: / Line 150: @@
 [[Image:PCR131517P.jpg]]
-Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]][[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]]expected product size:  bp
+Primer used:[[Team:LMU-Munich/Schedule/pduA_fwd|pduA fwd]],[[Team:LMU-Munich/Schedule/pduU_rev|pduU rev]] expected product size:  bp
-==In Vitro testing of the chemical kinetics with the enzymes==
-==Transforming the construct in bacteria==
-==Testing the system==
 {{:Team:LMU-Munich/Templates/Page Footer}}