"

From 2010.igem.org

Revision as of 22:21, 30 June 2010 (view source) Kevijose (Talk | contribs) ← Older edit		Revision as of 22:22, 30 June 2010 (view source) Kevijose (Talk | contribs) Newer edit →
Line 16:		Line 16:
	|[[Team:Michigan/Pili_Expression#6/28/2010|6/28/2010]]		|[[Team:Michigan/Pili_Expression#6/28/2010|6/28/2010]]
	|[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]]		|[[Team:Michigan/Pili_Expression#6/29/2010|6/29/2010]]
-	| -	+	|[[Team:Michigan/Pili_Expression#6/30/2010|6/30/2010]]
	| -		| -
	| -		| -

---

Revision as of 22:22, 30 June 2010

	Sunday	Monday	Tuesday	Wednesday	Thursday	Friday	Saturday
Week 1	-	6/28/2010	6/29/2010	6/30/2010	-	-	-

6/28/2010

Made a 500 mL batch of LB broth

• When pouring in distilled water, pour a few mL at a time to avoid clumping.
• It takes 20g of powder to make 1 L of broth

Sterilized broth using the autoclave

• The temperature setting on the autoclave is off by a little bit
• Set dial 2 notches below 134 °C.

6/29/2010

Started growing E. coli K12 cultures

• Poured 2 mL of LB broth into a Falcon tube
• Used strain of K12 from Dr. Lin's freezer
• Placed in incubator shaker for 24 hrs.

Added and inventoried supplies from Dr. Pinto's lab.

• Regular trash can be thrown out by going down one floor, then going outside to the trash bins.
• Chemical wastes must be cleaned by calling OSEH.

6/30/2010

Cryopreserved stock of K12

• 1 mL located in the iGEM box in the Lin lab.
• 1 mL located in the lab freezer.