Team:UNIPV-Pavia/Calendar/October/settimana3

From 2010.igem.org

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In order to obtain better results with screening PCR for the E. coli genome integration project, we perform amplifications using an annealing temperature of 63°C.
 
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Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976, BBa_K300976-BBa_K300977, BBa_K300977-BBa_K300978.
 
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[[Image:UNIPV10_19-10-10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTO_P1_P2).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976.]]
 
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==October, 19th==
==October, 19th==
<b>Wiki update and definition of results.</b>
<b>Wiki update and definition of results.</b>
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 +
----
 +
Every clones of E. coli genome integration project were amplified with PCR to verify their lengths and their positions into the genome.
 +
In order to obtain better results we perform all the amplifications using an annealing temperature of 63°C.
 +
Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976 (P1-P2), BBa_K300976-BBa_K300977 (P2-P3), BBa_K300977-BBa_K300978 (P3-P4).
 +
 +
[[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P1-P2).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976 (P1-P2).]]
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[[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P2-P3).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300976-BBa_K300977 (P2-P3).]]
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[[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P3-P4).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300977-BBa_K300978 (P3-P4).]]
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<div align="right"><small>[[#indice|^top]]</small></div>
<div align="right"><small>[[#indice|^top]]</small></div>

Latest revision as of 10:09, 25 October 2010


OCTOBER: WEEK 3



October, 18th

We have finally completed the parts transfer to <partinfo>pSB1C3</partinfo> plasmid. All the parts that will be sent to the registry were previously screened by UNIPV-Pavia TEAM, as reported in this notebook and all of them were sequenced and sequencing results were correct.

After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis.

The parts we sent to MIT and submitted to the registry are:

Part nameWiki namePlasmidNumber
<partinfo>BBa_K300005</partinfo>I0pSB1C31
<partinfo>BBa_K300006</partinfo>I1pSB1C32
<partinfo>BBa_K300009</partinfo>I3pSB1C33
<partinfo>BBa_K300008</partinfo>I5pSB1C34
<partinfo>BBa_K300024</partinfo>I7pSB1C35
<partinfo>BBa_K300019</partinfo>I12pSB1C36
<partinfo>BBa_K300030</partinfo>I14pSB1C37
<partinfo>BBa_K300028</partinfo>I15pSB1C38
<partinfo>BBa_K300029</partinfo>I16pSB1C39
<partinfo>BBa_K300025</partinfo>I17pSB1C310
<partinfo>BBa_K300026</partinfo>I18pSB1C311
<partinfo>BBa_K300027</partinfo>I19pSB1C312
<partinfo>BBa_K300002</partinfo>I20pSB1C313
<partinfo>BBa_K300003</partinfo>I21pSB1C314
<partinfo>BBa_K300031</partinfo>I22pSB1C315
<partinfo>BBa_K300081</partinfo>I26pSB1C316
<partinfo>BBa_K300079</partinfo>I31pSB1C317
<partinfo>BBa_K300080</partinfo>I35pSB1C318
<partinfo>BBa_K300086</partinfo>I47pSB1C319
<partinfo>BBa_K300088</partinfo>I48pSB1C320
<partinfo>BBa_K300090</partinfo>I49pSB1C321
<partinfo>BBa_K300082</partinfo>I55pSB1C322
<partinfo>BBa_K300084</partinfo>I78pSB1C323
<partinfo>BBa_K300083</partinfo>I79pSB1C324
<partinfo>BBa_K300010</partinfo>I80pSB1C325
<partinfo>BBa_K300007</partinfo>I70 - BioBrick<partinfo>BBa_K300001</partinfo>26
<partinfo>BBa_K300007</partinfo>I70 - vector<partinfo>BBa_K300001</partinfo>27
<partinfo>BBa_K300004</partinfo>INTEINpSB1C328
<partinfo>BBa_I52002</partinfo>I51<partinfo>BBa_K300000</partinfo>29


The parts we have in freezer and we only submitted to the Registry are:

Part nameWiki namePlasmid
<partinfo>BBa_K300021</partinfo>I8<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300022</partinfo>I9<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300023</partinfo>I10<partinfo>BBa_J61002</partinfo>
<partinfo>BBa_K300091</partinfo>I63<partinfo>pSB1AK3</partinfo>
<partinfo>BBa_K300092</partinfo>I64<partinfo>pSB1A2</partinfo>
<partinfo>BBa_K300099</partinfo>I65<partinfo>pSB1A2</partinfo>
<partinfo>BBa_K300980</partinfo>YEAST MR.GpMA commercial vector
<partinfo>BBa_K300982</partinfo>CREAM-
<partinfo>BBa_K300983</partinfo>CRIM MR.GpMK-RQ commercial vector




October, 19th

Wiki update and definition of results.


Every clones of E. coli genome integration project were amplified with PCR to verify their lengths and their positions into the genome. In order to obtain better results we perform all the amplifications using an annealing temperature of 63°C. Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976 (P1-P2), BBa_K300976-BBa_K300977 (P2-P3), BBa_K300977-BBa_K300978 (P3-P4).

The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976 (P1-P2).
The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300976-BBa_K300977 (P2-P3).
The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300977-BBa_K300978 (P3-P4).



October, 20th

Wiki update and definition of results.

October, 21st

Wiki update and definition of results.

October, 22nd

Wiki update and definition of results.

October, 23rd

Wiki update and definition of results.