Team:UNIPV-Pavia/Calendar/October/settimana3
From 2010.igem.org
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(→October, 19th) |
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<tr><td><partinfo>BBa_K300091</partinfo></td><td>I63</td><td><partinfo>pSB1AK3</partinfo></td></tr> | <tr><td><partinfo>BBa_K300091</partinfo></td><td>I63</td><td><partinfo>pSB1AK3</partinfo></td></tr> | ||
<tr><td><partinfo>BBa_K300092</partinfo></td><td>I64</td><td><partinfo>pSB1A2</partinfo></td></tr> | <tr><td><partinfo>BBa_K300092</partinfo></td><td>I64</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
+ | <tr><td><partinfo>BBa_K300099</partinfo></td><td>I65</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
<tr><td><partinfo>BBa_K300980</partinfo></td><td>YEAST MR.G</td><td>pMA commercial vector</td></tr> | <tr><td><partinfo>BBa_K300980</partinfo></td><td>YEAST MR.G</td><td>pMA commercial vector</td></tr> | ||
<tr><td><partinfo>BBa_K300982</partinfo></td><td>CREAM</td><td>-</td></tr> | <tr><td><partinfo>BBa_K300982</partinfo></td><td>CREAM</td><td>-</td></tr> | ||
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==October, 19th== | ==October, 19th== | ||
<b>Wiki update and definition of results.</b> | <b>Wiki update and definition of results.</b> | ||
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+ | ---- | ||
+ | Every clones of E. coli genome integration project were amplified with PCR to verify their lengths and their positions into the genome. | ||
+ | In order to obtain better results we perform all the amplifications using an annealing temperature of 63°C. | ||
+ | Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976 (P1-P2), BBa_K300976-BBa_K300977 (P2-P3), BBa_K300977-BBa_K300978 (P3-P4). | ||
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+ | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P1-P2).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976 (P1-P2).]] | ||
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+ | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P2-P3).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300976-BBa_K300977 (P2-P3).]] | ||
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+ | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P3-P4).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300977-BBa_K300978 (P3-P4).]] | ||
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<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Latest revision as of 10:09, 25 October 2010
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