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Team:UNIPV-Pavia/Calendar/October/settimana3
From 2010.igem.org
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After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis. | After parts have been moved to the shipping plasmid and checked by agarose gel electrophoresis. | ||
| - | The parts we sent to MIT are: | + | The parts we sent to MIT and submitted to the registry are: |
<table border='1' align='center'> | <table border='1' align='center'> | ||
| Line 72: | Line 72: | ||
<tr><td><partinfo>BBa_K300004</partinfo></td><td>INTEIN</td><td>pSB1C3</td><td>28</td></tr> | <tr><td><partinfo>BBa_K300004</partinfo></td><td>INTEIN</td><td>pSB1C3</td><td>28</td></tr> | ||
<tr><td><partinfo>BBa_I52002</partinfo></td><td>I51</td><td><partinfo>BBa_K300000</partinfo></td><td>29</td></tr> | <tr><td><partinfo>BBa_I52002</partinfo></td><td>I51</td><td><partinfo>BBa_K300000</partinfo></td><td>29</td></tr> | ||
| + | </table> | ||
| + | |||
| + | The parts we have in freezer and we only submitted to the Registry are: | ||
| + | <table border='1' align='center'> | ||
| + | <tr><th>Part name</th><th>Wiki name</th><th>Plasmid</th></tr> | ||
| + | <tr><td><partinfo>BBa_K300021</partinfo></td><td>I8</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300022</partinfo></td><td>I9</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300023</partinfo></td><td>I10</td><td><partinfo>BBa_J61002</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300091</partinfo></td><td>I63</td><td><partinfo>pSB1AK3</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300092</partinfo></td><td>I64</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300099</partinfo></td><td>I65</td><td><partinfo>pSB1A2</partinfo></td></tr> | ||
| + | <tr><td><partinfo>BBa_K300980</partinfo></td><td>YEAST MR.G</td><td>pMA commercial vector</td></tr> | ||
| + | <tr><td><partinfo>BBa_K300982</partinfo></td><td>CREAM</td><td>-</td></tr> | ||
| + | <tr><td><partinfo>BBa_K300983</partinfo></td><td>CRIM MR.G</td><td>pMK-RQ commercial vector</td></tr> | ||
| + | <!--tr><td><partinfo></partinfo></td><td></td><td><partinfo></partinfo></td></tr--> | ||
</table> | </table> | ||
| + | |||
| + | ---- | ||
| Line 80: | Line 97: | ||
==October, 19th== | ==October, 19th== | ||
| - | Wiki update and definition of results. | + | <b>Wiki update and definition of results.</b> |
| + | |||
| + | ---- | ||
| + | Every clones of E. coli genome integration project were amplified with PCR to verify their lengths and their positions into the genome. | ||
| + | In order to obtain better results we perform all the amplifications using an annealing temperature of 63°C. | ||
| + | Screening PCR was performed on MC42(A-B-C), MC43(A-B-C), MG42(B-C), MG43(A-B-C), MC1061 and MG1655. Primers used were BBa_K300975-BBa_K300976 (P1-P2), BBa_K300976-BBa_K300977 (P2-P3), BBa_K300977-BBa_K300978 (P3-P4). | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P1-P2).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300975-BBa_K300976 (P1-P2).]] | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P2-P3).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300976-BBa_K300977 (P2-P3).]] | ||
| + | |||
| + | [[Image:UNIPV10_19_10_10_MC42ABC_MC43ABC_MG42BC_MG43ABC_BLANK_MC1061_MG1655(TUTTI P3-P4).jpg|thumb|300px|center|The order of the samples was the follow: MC42(A,B,C), MC43(A,B,C), MG42(B,C), MG43(A,B,C), blank, MC1061, MG1655. Primers were BBa_K300977-BBa_K300978 (P3-P4).]] | ||
| + | |||
| + | |||
| + | |||
| + | |||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 20th== | ==October, 20th== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 21st== | ==October, 21st== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 22nd== | ==October, 22nd== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
==October, 23rd== | ==October, 23rd== | ||
| - | + | <b>Wiki update and definition of results.</b> | |
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Latest revision as of 10:09, 25 October 2010
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