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Team:Tsinghua/Notebook/5 August 2010
From 2010.igem.org
(Difference between revisions)
(New page: == Module I, DT and Fan's part: == pBMN-PIB plasmid purification. == result == No colony with pKD13 or pKD3 exists. It is said that these two plasmids also have the Amp resistant gene. ...) |
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| - | + | == Module I, group 2(b) == | |
| - | == Module I, | + | |
pBMN-PIB plasmid purification. | pBMN-PIB plasmid purification. | ||
| - | == result == | + | === result === |
No colony with pKD13 or pKD3 exists. It is said that these two plasmids also have the Amp resistant gene. We’ll try kan and chlr antibiotic to select next time. | No colony with pKD13 or pKD3 exists. It is said that these two plasmids also have the Amp resistant gene. We’ll try kan and chlr antibiotic to select next time. | ||
| + | |||
| + | == Module I, YX and ZY's part: == | ||
| + | Wonderful! | ||
| + | Colonies grew on the plates! | ||
| + | We tipped the colonies into 2ml LB media and cultured them on a 37 centigrade shaker. | ||
| + | We'll extract plasmids from them later. | ||
Latest revision as of 06:53, 25 October 2010
Module I, group 2(b)
pBMN-PIB plasmid purification.
result
No colony with pKD13 or pKD3 exists. It is said that these two plasmids also have the Amp resistant gene. We’ll try kan and chlr antibiotic to select next time.
Module I, YX and ZY's part:
Wonderful! Colonies grew on the plates! We tipped the colonies into 2ml LB media and cultured them on a 37 centigrade shaker. We'll extract plasmids from them later.
