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Team:Tsinghua/Notebook/4 August 2010
From 2010.igem.org
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| - | == Module I, | + | == Module I, group 2(b) == |
Use the series-1 primers to PCR the fragments in SuperMix system. Purify and measure the concentration. | Use the series-1 primers to PCR the fragments in SuperMix system. Purify and measure the concentration. | ||
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SuperMix 25μl | SuperMix 25μl | ||
Total 50μl | Total 50μl | ||
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| + | == Module I, YX and ZY's part == | ||
| + | We transformed competent E. coli cell, trans5a and placed the cells onto LB media with Kanamycin. | ||
| + | Waiting for the colonies to grow! Excited! | ||
Latest revision as of 06:52, 25 October 2010
SuperMixII
Wonderful!
Module I, group 2(b)
Use the series-1 primers to PCR the fragments in SuperMix system. Purify and measure the concentration.
PCR system (SuperMix):
H2O 22μl primer1 1μl primer2 1μl template 1μl SuperMix 25μl Total 50μl
Module I, YX and ZY's part
We transformed competent E. coli cell, trans5a and placed the cells onto LB media with Kanamycin. Waiting for the colonies to grow! Excited!
