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Team:Tsinghua/Notebook/27 July 2010
From 2010.igem.org
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Using the newly synthesized primers go through a pre-test experiment. | Using the newly synthesized primers go through a pre-test experiment. | ||
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FastPFU 0.6μl | FastPFU 0.6μl | ||
Total 50μl | Total 50μl | ||
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== Module I, Danyang's part: == | == Module I, Danyang's part: == | ||
Revision as of 06:51, 25 October 2010
Module I, group 2(b)
Using the newly synthesized primers go through a pre-test experiment.
PCR system (FastPFU):
H2O 31.4μl 5×buffer 10μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.6μl Total 50μl
Module I, Danyang's part:
Conduct PCR to amplify eGFP, Kan and Chlr with DNA polymerase pyrobest.
PCR system (FastPFU):
H2O 36.7μl 10×buffer 5μl dNTP 5μl primer1 1μl primer2 1μl template 1μl FastPFU 0.3μl Total 50μl
result
The concentration of the M, K and C are a little lower. We’ll amplify them once again with two degrees lower of the annealing temperature.
