Latest revision as of 03:09, 25 October 2010

Week #2 13th September - 17th 2010 Holidays in México

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-= Week 2   13th  September - 17th  2010 Holidays in México =
+= Week #2   13th  September - 17th  2010 Holidays in México =
-= Week 3  18th September -22th 2010 =
-==''Monday''==
-==='''Planned and prepared for tomorrow's electroporation'''===
-*'''Depart electroporator.'''
-*'''Made up LB agar plates and cloramphenicol 100μl.'''
-*'''Plan to transform Psb1C3 froma plate.'''
-==='''We were looking for a nanodrop to quantify DNA’s concentrations'''===
-==='''and tried to make ligations Psb1C3 with each one of primers'''===
-==='''(our this year's bricks).'''===
-==''Tuesday''==
-==='''Transformed Top 10 competent cells, and did 10 plates incubated overnight.'''===
-==''Wednsday''==
-==='''Results previous day transformations only two plates were completed unsucessful.'''===
-*'''Prepared solutions for miniprep via Alcalin lisis method'''
-[[Image:Imagen2.gif]]
-==''Thursday''==
-==='''The inoculum for miniprep we prepared 6 vector’s vials'''===
-==='''without positive results. Maybe the solutions for alcaline'''===
-==='''lisis are not well.'''===
-[[Image:Imagen3.gif|500px]]
-*'''We have discused over how to quantify our cold sistem response'''
-'''we gonig to use a promoter sistem, the funcional casset   using  J13002  as follow'''
-*'''Promoter + our functional secuence + GFP'''
-[[Image:Cuant1.gif]]
-*'''Promoter + our functional secuence + AFP (Anty freeze protein)
-[[Image:Imagen4.gif]]