Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/14

From 2010.igem.org

(Difference between revisions)
(2010/10/14)
 
(One intermediate revision not shown)
Line 1: Line 1:
 +
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
=2010/10/14=
=2010/10/14=

Latest revision as of 02:59, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/10/14

1:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 14hr 29min.JPG


2:DNA extraction

<Member>
nito

<Sample>

<Protocol>
See Protocol 4


3:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10


4:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

  • plasmid

・Digest enzyme

  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


5:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


6:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4


7:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions

・Digest enzyme

  • AvrⅡ
  • NheⅠ
  • XbaⅠ
  • SpeⅠ

<Protocol>
See Prptpcpl 9


8:PCR

<Member>
nito

<Sample>

<Protocol>
See Protocol 1


9:DNA extraction from gels

<Member>
nito

<Sample>

  • 9-7

(After electrophoresis)


<Protocol>
SeeProtocol 4


10:Electrophoresis

<Member>
nito

<Sample>

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-14 22hr 56min.JPG


11:DNA extraction from gels

<Member>
nito

<Sample>

(After electrophoresis)

<Protocol>
SeeProtocol 4