Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/10

From 2010.igem.org

(Difference between revisions)
(3:DNA Digestion)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
{{:Team:Tokyo_Metropolitan/Header}}
+
{{:Team:Tokyo_Metropolitan/Notebook/Pattern}}
 +
 
=2010/10/10=
=2010/10/10=
Line 55: Line 56:
<Sample><br />
<Sample><br />
-
*1-2   
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2]  
-
*10-2  
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
(after digestion)
(after digestion)
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ]
-
 
===5:DNA extraction===
===5:DNA extraction===
Line 69: Line 69:
<Sample><br />
<Sample><br />
PCR products
PCR products
-
*2
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
-
*4
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4]
-
*8
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8]
-
*10
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10]
-
*5
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
-
 
===6:Plasmid extraction from E-coli===
===6:Plasmid extraction from E-coli===
Line 84: Line 83:
<Sample><br />
<Sample><br />
-
*12
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 12]
-
*13
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 13]
-
*15
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 15]
-
*16
+
*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 16]
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Plasmid_extraction_from_E-coli/ Protocol 6 ]

Latest revision as of 02:58, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30 31
September 2010
SUNMONTUEWEDTHUFRISAT
1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30
October 2010
SUNMONTUEWEDTHUFRISAT
1 2
3 4 5 6 7 8 9
10 11 12 13 14 15 16
17 18 19 20 21 22 23
24 25 26 27 28 29 30
31

Contents

2010/10/10

1:Electrophoresis

<Member>
nito

<Sample>
PCR products (10/10)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-10 11hr 40min.JPG

<Result>
Matsuura 2010-10-10 21hr 24min.JPG

<Result>
Matsuura 2010-10-10 18hr 40min.JPG

<Result>
File:Image:Matsuura 2010-10-10 21hr 24min.JPG


2:PCR

<Member>
nito

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

3:DNA Digestion

<Member>
nito

<Sample, Materials>
・PCR productions


<Protocol>
See Prptpcpl 9

4:DNA Ligation

<Member>
nito

<Sample>

(after digestion)

<Protocol>
See Protocol 10

5:DNA extraction

<Member>
Mariko,mio,nito

<Sample>
PCR products

<Protocol>
See Protocol 4

6:Plasmid extraction from E-coli

<Member>
nito

<Sample>

<Protocol>
See Protocol 6