Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/09

From 2010.igem.org

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<Sample>(temperature in annealing:℃)<br />
<Sample>(temperature in annealing:℃)<br />
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ]
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===2:Electrophoresis①===
===2:Electrophoresis①===
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<Sample><br />
<Sample><br />
PCR products
PCR products
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2]
<Protocol><br />
<Protocol><br />
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<Result><br />
<Result><br />
[[Image:Matsuura_2010-10-09_18hr_03min.JPG]]
[[Image:Matsuura_2010-10-09_18hr_03min.JPG]]
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[[Image:Matsuura_2010-10-09_18hr_07min.JPG]]
===4:Electrophoresis③===
===4:Electrophoresis③===
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<Result><br />
<Result><br />
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[[Image:Matsuura_2010-10-09_18hr_07min.JPG]]
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[[Image:Matsuura_2010-10-09_18hr_25min.JPG]]
===5:Electrophoresis④===
===5:Electrophoresis④===
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<Sample><br />
<Sample><br />
PCR products
PCR products
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2]
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<Protocol><br />
<Protocol><br />
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<Result><br />
<Result><br />
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[[Image:Matsuura_2010-10-09_23hr_03min.JPG]]
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[[Image:Matsuura_2010-10-10_13hr_33min.JPG]]
===6:DNA Digestion===
===6:DNA Digestion===
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<Sample, Materials><br />
<Sample, Materials><br />
・PCR productions
・PCR productions
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*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1]
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*vector
*vector
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・Digest enzyme
 
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*Avr2
 
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*Nhe1
 
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*Spe1
 
<Protocol><br />
<Protocol><br />
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ]

Latest revision as of 02:58, 25 October 2010


E.coli Pattern Formation Project Notebook



August 2010
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Contents

2010/10/09

1:PCR

<Member>
Hitomi

<Sample>(temperature in annealing:℃)

<Protocol>
See Protocol 1

2:Electrophoresis①

<Member>
Hitomi

<Sample>
PCR products

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 14hr 11min.JPG

3:Electrophoresis②

<Member>
Hitomi

<Sample>
PCR products (10/8)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 18hr 03min.JPG

Matsuura 2010-10-09 18hr 07min.JPG

4:Electrophoresis③

<Member>
Hitomi

<Sample>
PCR products (10/9)

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 18hr 25min.JPG

5:Electrophoresis④

<Member>
Mariko

<Sample>
PCR products

<Protocol>
See Protocol 8

<Result>
Matsuura 2010-10-09 23hr 03min.JPG

Matsuura 2010-10-10 13hr 33min.JPG

6:DNA Digestion

<Member>
Mariko

<Sample, Materials>
・PCR productions

<Protocol>
See Prptpcpl 9