"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/09
From 2010.igem.org
(Difference between revisions)
(New page: {{:Team:Tokyo_Metropolitan/Header}} =2010/10/09= ===1:PCR=== <Member><br /> Hitomi <Sample>(temperature in annealing:℃)<br /> *1 *2 *3 *4 *5 *6 *7 *8 *9 *10 *1-2 *2-3 *2-7 *2-10 *4-5 *...) |
|||
| (13 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
| + | |||
=2010/10/09= | =2010/10/09= | ||
| Line 7: | Line 8: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
| - | *1-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1-2] |
| - | *2-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-3] |
| - | *2-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-7] |
| - | *2-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
| - | *6-3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-3] |
| - | *6-7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6-7] |
| - | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Pho_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 1 ] | ||
| - | |||
===2:Electrophoresis①=== | ===2:Electrophoresis①=== | ||
| Line 36: | Line 36: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *10-2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10-2] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 42: | Line 42: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-09_14hr_11min.JPG]] |
| - | + | ||
===3:Electrophoresis②=== | ===3:Electrophoresis②=== | ||
| Line 56: | Line 55: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-09_18hr_03min.JPG]] |
| + | [[Image:Matsuura_2010-10-09_18hr_07min.JPG]] | ||
===4:Electrophoresis③=== | ===4:Electrophoresis③=== | ||
| Line 70: | Line 70: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-09_18hr_25min.JPG]] |
| - | + | ||
===5:Electrophoresis④=== | ===5:Electrophoresis④=== | ||
| Line 79: | Line 78: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] |
| - | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
| - | *7 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7] |
| - | *8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] |
| - | *9 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] |
| - | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
| - | *2-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2-10] |
| - | *4-5 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4-5] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 96: | Line 95: | ||
<Result><br /> | <Result><br /> | ||
| - | [[Image:]] | + | [[Image:Matsuura_2010-10-09_23hr_03min.JPG]] |
| + | [[Image:Matsuura_2010-10-10_13hr_33min.JPG]] | ||
===6：DNA Digestion=== | ===6：DNA Digestion=== | ||
| Line 105: | Line 105: | ||
＜Sample, Materials＞<br /> | ＜Sample, Materials＞<br /> | ||
・PCR productions | ・PCR productions | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *3 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 3] |
| - | *4 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] |
| - | *6 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 6] |
| - | *10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10] |
| - | *7-8-10 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8-10] |
*vector | *vector | ||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Digestion/ Prptpcpl 9 ] | ||
Latest revision as of 02:58, 25 October 2010
E.coli Pattern Formation Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/10/09
1:PCR
<Member>
Hitomi
<Sample>(temperature in annealing:℃)
<Protocol>
See Protocol 1
2:Electrophoresis①
<Member>
Hitomi
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
3:Electrophoresis②
<Member>
Hitomi
<Sample>
PCR products (10/8)
<Protocol>
See Protocol 8
<Result>
4:Electrophoresis③
<Member>
Hitomi
<Sample>
PCR products (10/9)
<Protocol>
See Protocol 8
<Result>
5:Electrophoresis④
<Member>
Mariko
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
6：DNA Digestion
＜Member＞
Mariko
＜Sample, Materials＞
・PCR productions
＜Protocol＞
See Prptpcpl 9
