"
Team:Tokyo Metropolitan/Notebook/Pattern/2010/10/02
From 2010.igem.org
(Difference between revisions)
(→3:Electrophoresis) |
|||
| (4 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:Tokyo_Metropolitan/ | + | {{:Team:Tokyo_Metropolitan/Notebook/Pattern}} |
| + | |||
=2010/10/02= | =2010/10/02= | ||
| Line 8: | Line 9: | ||
<Sample>(temperature in annealing:℃)<br /> | <Sample>(temperature in annealing:℃)<br /> | ||
Transformation 9/28 | Transformation 9/28 | ||
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ] | ||
| - | |||
===2：DNA Ligation === | ===2：DNA Ligation === | ||
| Line 20: | Line 20: | ||
＜Sample＞<br /> | ＜Sample＞<br /> | ||
| - | *1 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] |
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
(after digestion) | (after digestion) | ||
＜Protocol＞<br /> | ＜Protocol＞<br /> | ||
See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 10 ] | ||
| - | |||
===3:Electrophoresis=== | ===3:Electrophoresis=== | ||
| Line 34: | Line 33: | ||
<Sample><br /> | <Sample><br /> | ||
PCR products | PCR products | ||
| - | *2 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] |
| - | *7-8 | + | *[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 7-8] |
<Protocol><br /> | <Protocol><br /> | ||
| Line 49: | Line 48: | ||
[[Image:Matsuura_2010-10-02_20hr_46min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_20hr_46min.JPG]]<br /> | ||
| - | [[Image:Matsuura_2010-10- | + | [[Image:Matsuura_2010-10-02_22hr_42min.JPG]]<br /> |
[[Image:Matsuura_2010-10-02_22hr_43min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_22hr_43min.JPG]]<br /> | ||
| Line 57: | Line 56: | ||
[[Image:Matsuura_2010-10-02_21hr_41min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_21hr_41min.JPG]]<br /> | ||
| - | [[Image:Matsuura_2010-10- | + | [[Image:Matsuura_2010-10-02_22hr_13min.JPG]]<br /> |
[[Image:Matsuura_2010-10-02_23hr_30min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_23hr_30min.JPG]]<br /> | ||
[[Image:Matsuura_2010-10-02_23hr_31min.JPG]]<br /> | [[Image:Matsuura_2010-10-02_23hr_31min.JPG]]<br /> | ||
Latest revision as of 02:55, 25 October 2010
E.coli Pattern Formation Project Notebook
|
|
|
||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Contents |
2010/10/02
1:PCR
<Member>
mio,mizuki
<Sample>(temperature in annealing:℃)
Transformation 9/28
<Protocol>
See Protocol 2
2：DNA Ligation
＜Member＞
mizuki
＜Sample＞
(after digestion)
＜Protocol＞
See Protocol 10
3:Electrophoresis
<Member>
mio,mizuki
<Sample>
PCR products
<Protocol>
See Protocol 8
<Result>
