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Revision as of 15:21, 29 June 2010 (view source) Timsterxox (Talk | contribs) ← Older edit		Revision as of 16:19, 29 June 2010 (view source) Gcxmatt (Talk | contribs) (→Reagents) Newer edit →
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-	==Reagents==	+	=='''Reagents'''==
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-	[[Reagents]]	+
	=='''Cell Staining'''==		=='''Cell Staining'''==
	===[[Rhodamine-Phalloidin/Calcofluor Staining]]===		===[[Rhodamine-Phalloidin/Calcofluor Staining]]===
	===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===		===[[LIVE/DEAD® BacLight - Bacterial Viability Kit]]===

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Revision as of 16:19, 29 June 2010

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Contents 1 DNA 1.1 Prepping DNA from the kit plates 1.2 Quikchange (from primers to colonies!) 1.3 Kit to Stock Plasmid 2 Bacterial Work 2.1 Transformation 2.2 O/N Culture 2.3 Preparation of Competent Cells 2.4 LB Media 2.5 Preparing Plates 3 Assembly 3.1 Restriction Enzyme Digests 3.2 3A Assembly 3.3 Ligations 3.4 Mini Prep 4 Microscopy 4.1 Confocal Microscopy 4.2 Fluorescence Microscopy 5 Reagents 6 Cell Staining 6.1 Rhodamine-Phalloidin/Calcofluor Staining 6.2 LIVE/DEAD® BacLight - Bacterial Viability Kit

DNA

Prepping DNA from the kit plates

Quikchange (from primers to colonies!)

Kit to Stock Plasmid

Bacterial Work

Transformation

O/N Culture

Preparation of Competent Cells

LB Media

Preparing Plates

Assembly

Restriction Enzyme Digests

3A Assembly

Ligations

Mini Prep

Microscopy

Confocal Microscopy

Fluorescence Microscopy

Reagents

Cell Staining

Rhodamine-Phalloidin/Calcofluor Staining

LIVE/DEAD® BacLight - Bacterial Viability Kit