Team:Aberdeen Scotland/Evolution
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Revision as of 21:37, 24 October 2010
University of Aberdeen - ayeSwitch
What is directed evolution?
In protein engineering, the method of directed evolution is used to harness the power of natural selection. This is to evolve certain proteins or RNA with desirable properties not found in nature. Rather than swapping out different components of the structure, directed evolution can be used to manipulate the behaviour of these components. Then, by screening or selection, we can obtain the desired phenotype.
There are three typical steps involved that include:
1. Diversification: The gene that encodes the desired protein is mutated and recombined at random to create a large library of gene mutants. Techniques commonly used in this step involve PCR and DNA shuffling.
2. Selection: The library is tested for the existence of mutants possessing the property of interest using screening or selection.
a. Selections automatically remove all mutants that do not function.
b. Screening enables the researcher to manually detect and isolate the high-performing mutants.
3. Amplifications: The mutations identified int he selection or screening process are replicated several times so it allows the researchers to sequence their DNA and recognize what mutations have occurred.
For optimizing efficiency, altering more than one parameter would be preferable in order to obtain the desired protein. Therefore, we accululate mutations over multiple generations.
In order to examine how changes in the parameters were affecting the stability of the steady states, we could do uniform bifurcation analysis e.g. determining th regions of stability.
In the experiments, we were able to use the FACS (fluorescence activated cells sorting) machine to separate the proteins with the brightest colours and thus use the strongest ones. Once we had this, we could put the proteins through the system again as initial conditions and then we would have a more distinctive switch.
![](https://static.igem.org/mediawiki/2010/3/3a/FACS.gif)
Pros
Directed evolution can systematically perturb and distinguish components then, using these perturbed components, we can gain further insight into the functionality of the normal circuit.
If some of the pathways enhance the desired performance, then iterative rounds of random mutation of the circuit and screening for the desired properties may provide steady improvement. This only works if the initial design is close to a better design.
Why use maths to model directed evolution?
There are three primary limitations that mathematical modelling can overcome:
1. The evolutionary space for a genetic circuit is too large to explore efficiently.
2. Refining our parameters is much easier than improving (or altering) the system experimentally.
3. Although selecting for independent properties is possible, it usually requires setting up multiple rounds of screening or selection.
Procedure
1. We first took the basic program where we integrated the system, plugging in an estimation of the parameters.
2. For each parameter, it yields the value for which we obtain the largest difference between GFP and CFP.
3. We then fix out chosen parameter and continue the program with the next parameter.
4. This can be done over and over for each parameter of the model, eventually producing a clearly defined switch that will somewhat resemble our original system.
5. Repeat these steps for each parameter in the system to eventually produce a clearly optimized switch that will yield the maximum difference between GFP and CFP when switching from one state to the other.
Results
Fig 1. shows the behaviour of the original system over a set period of time.
![](https://static.igem.org/mediawiki/2010/3/38/Initial.jpg)
Fig 1.
As shown, the system has been integrated over a long enough timespan such that it has reached equilibrium.
Once we had this, we could then do this again but change one parameter and instead insert a range of values that can be put through the system.
Then we found the value for that parameter that produced the biggest switch between the two proteins. We did this for each parameter until we had an “ideal” value for each one. Finally, we entered these values into the system and integrated it again.
![](https://static.igem.org/mediawiki/2010/6/6e/Final.jpg)
Fig 2.
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