Team:Groningen/Biobricks
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== Biobricks == | == Biobricks == | ||
+ | Here is our registry of our biobricks: | ||
<groupparts>iGEM010 Groningen</groupparts> | <groupparts>iGEM010 Groningen</groupparts> | ||
+ | |||
+ | '''Summary:''' | ||
+ | |||
+ | Below is given a short description of all the biobricks in our registry. | ||
+ | |||
+ | |||
+ | '''Chaplins''' | ||
+ | |||
+ | BBa_K305001 Hydrophobic protein chaplin C (chpC) | ||
+ | |||
+ | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
+ | |||
+ | BBa_K305002 chaplin C (chpDC) with modified sortase binding motive | ||
+ | |||
+ | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
+ | |||
+ | Modified C-terminal Sortase(SrtA from S. aureus.) recognition site. | ||
+ | |||
+ | BBa_K305003 Hydrophobic protein chaplin E (chpE) | ||
+ | |||
+ | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
+ | |||
+ | BBa_K305004 Hydrophobic protein chaplin H (chpH) | ||
+ | |||
+ | Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis. | ||
+ | |||
+ | BBa_K305005Sortase srtA | ||
+ | |||
+ | Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall | ||
+ | |||
+ | |||
+ | |||
+ | '''Promotors''' | ||
+ | |||
+ | BBa_K305006 yqxMpr | ||
+ | |||
+ | yqxM promotor effective in biofilm formation, regulates tasA production | ||
+ | |||
+ | BBa_K305007 srfApr 1 | ||
+ | |||
+ | Promotor for the surfactin regulatory gene. | ||
+ | |||
+ | BBa_K305008 srfApr 2 | ||
+ | |||
+ | Promotor for the surfactin regulatory gene. | ||
+ | |||
+ | BBa_K305009 srfApr1>GFP | ||
+ | |||
+ | Promotor for the surfactin regulatory gene with GFP. | ||
+ | |||
+ | BBa_K305010 srfApr2>GFP | ||
+ | |||
+ | Promotor for the surfactin regulatory gene with GFP. | ||
+ | |||
+ | |||
+ | |||
+ | '''Plasmid Backbone''' | ||
+ | |||
+ | BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis | ||
+ | |||
+ | Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was | ||
+ | constructed to express proteins present in RCF 10 BioBricks upon subtilin induction. |
Revision as of 20:56, 24 October 2010
Biobricks
Here is our registry of our biobricks: <groupparts>iGEM010 Groningen</groupparts>
Summary:
Below is given a short description of all the biobricks in our registry.
Chaplins
BBa_K305001 Hydrophobic protein chaplin C (chpC)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305002 chaplin C (chpDC) with modified sortase binding motive
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
Modified C-terminal Sortase(SrtA from S. aureus.) recognition site.
BBa_K305003 Hydrophobic protein chaplin E (chpE)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305004 Hydrophobic protein chaplin H (chpH)
Strongly hydrophobic protein originating from Streptomyces coelicolor and codon optimized for Bacillus subtilis.
BBa_K305005Sortase srtA
Staphylococcus aureus sortase, an enzyme that anchors surface proteins to the cell wall
Promotors
BBa_K305006 yqxMpr
yqxM promotor effective in biofilm formation, regulates tasA production
BBa_K305007 srfApr 1
Promotor for the surfactin regulatory gene.
BBa_K305008 srfApr 2
Promotor for the surfactin regulatory gene.
BBa_K305009 srfApr1>GFP
Promotor for the surfactin regulatory gene with GFP.
BBa_K305010 srfApr2>GFP
Promotor for the surfactin regulatory gene with GFP.
Plasmid Backbone
BBa_K305011 Subtilin inducible expression plasmid for Bacillus subtilis
Adapted from the system for subtilin-regulated gene expression (SURE) in Bacillus subtilis [1], this plasmid was constructed to express proteins present in RCF 10 BioBricks upon subtilin induction.