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Team:UCL London/Digestion
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Latest revision as of 20:49, 24 October 2010
Restriction Enzyme Digestion
Materials
Plasmid DNA: 10.5 µL/5 µL
Restriction Enzymes (depend on the requirements of the parts)
EcoRI: 1 µL
PstI: 1 µL
SpeI: 1 µL
XbaI: 1 µL
10× NE buffer: 1.5 µL/1 µL (lid)
BSA: 1 µL (lid)
Method
1. Add all the contents listed above to labelled eppendorfs.
2. Spin for 30 sec.
3. Incubation at 37°C for 1hr.
4. Leave on ice before the gel electrophoresis
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